Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
Line 488: Line 488:
·      Colony PCR<br>
·      Colony PCR<br>
·      Extract gel B0015 & comED58E<br>
·      Extract gel B0015 & comED58E<br>
-
·   <br>
+
·     Mutagenesis attempts for <i>comE</i><br>
<br> <b> July 17</b> <br>
<br> <b> July 17</b> <br>
Line 627: Line 627:
·      Restrict check K880005 <br>
·      Restrict check K880005 <br>
·      Promoters colony PCR <br>
·      Promoters colony PCR <br>
-
·      <br>
+
·      PCR for <i>comE</i> fragments (mutagenesis) for Gibson Assembly<br>
-
·      <br>
+
 
-
·   <br>
+
 
<br> <b> July 30</b> <br>
<br> <b> July 30</b> <br>
Line 648: Line 648:
·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      K880005 dephosphorylation & ligation<br>
·      K880005 dephosphorylation & ligation<br>
-
·      Promoters Colony PCR + Sequencing reaction   <br>
+
·      Promoters Colony PCR + Sequencing reaction<br>
 +
·      PCR for <i>comED58E fragments (mutagenesis) for Gibson Assembly<br>
       </div>
       </div>

Revision as of 13:25, 17 October 2014



Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Project Pneuumonsensor and Project Riboregulator are displayed separately.






Pneumosensor

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5


TOP

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement