Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
Line 1,586: Line 1,586:
<br> <b> July 8 </b> <br>
<br> <b> July 8 </b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Transformation of ligated TAs and CRs<br>
-
·     <br>
+
· Miniprep previous TAs CRs constructs <br>
-
·     <br>
+
· Restrict check previous TAs CRs constructs <br>
-
·   <br>
+
· Colony PCR for CRs and TAs<br>
 +
. Re-ligation of TAs and CRs<br>
 +
. P<sub>bad</sub> PCR clean up and Digestion <br>
 +
. Ligation of P<sub>bad</sub> with pSB1C3<br>
 +
. Sequencing preparation of P<sub>bad</sub><br>
 +
 
<br> <b> July 9</b> <br>
<br> <b> July 9</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Transformation of Re-ligated TAs and CRs<br>
-
·     <br>
+
· Colony PCR<br>
-
·     <br>
+
· Gibson assembly master mix preparation<br>
-
·   <br>
+
 
<br> <b> July 10</b> <br>
<br> <b> July 10</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Sequencing preparation of P<sub>bad</sub><br>
-
·     <br>
+
· Miniprep previous TAs CRs constructs <br>
-
·     <br>
+
· Restrict check previous TAs CRs constructs <br>
-
·   <br>
+
· Colony PCR<br>
 +
. Inoculation for glycerol stocks <br>
 +
. Digest Receptor Backbone , digestion of BBa_ E0240 with EcoR1-HF and Xbal and gel purification <br>
 +
 
<br> <b> July 11</b> <br>
<br> <b> July 11</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Pbad digestion and gel purification, Digestion of PBAD-pSB1C3 with EcoR1-HF and Spe1 and gel purification <br>
-
·     <br>
+
· Ligation of pBAD and E0240<br>
-
·     <br>
+
· Overlaping  PCR design<br>
-
·   <br>
+
· Gibson assembly master mix preparation<br>
 +
. Glycerol stock<br>
 +
. Competent cells preparations for  DH5a and DH10B<br>
 +
. Run gel for yesterday's restriction check<br>
 +
. Miniprep  E0240, mRFP, B0015<br>
 +
. Colony PCR<br>
 +
. One full set re-ligation (short time) <br>
 +
. Miniprep I13401 and K592009<br>
       </div>
       </div>
   </div>
   </div>
Line 1,617: Line 1,632:
<br> <b> July 14 </b> <br>
<br> <b> July 14 </b> <br>
Wet lab<br>
Wet lab<br>
-
·      <br>
+
. Gibson assembly master mix preparation<br>
-
·      <br>
+
. Trevor's PCR<br>
-
·      <br>
+
. Ligation of pBAD an E0240<br>
-
·   <br>
+
. Competent cells preparations for  DH5a and DH10B<br>
-
 
+
. Prepare receptor backbone for oligo anealing, pSB1C3-J04450 (EcoRI, SpeI)<br>
 +
. One full set of re-ligation (short time) <br>
 +
. One full set of re-ligation (O/N) <br>
 +
. Preparations for Pbad re-Characterization <br>
<br> <b> July 15 </b> <br>
<br> <b> July 15 </b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Inoculation for pBAD-GFP-TT<br>
-
·     <br>
+
· (2nd time) Colony PCR usning VF2 and VR<br>
-
·     <br>
+
· Design primer for pBAD promoter sequencing <br>
-
·   <br>
+
· Gibson assembly master mix preparation<br>
 +
. Preparations for Pbad re-characterization <br>
 +
. Competent cells preparations for  DH5a and DH10B<br>
 +
. One full set re-ligation (O/N) <br>
 +
. Prepare receptor backbone for oligo anealing <br>
<br> <b> July 16</b> <br>
<br> <b> July 16</b> <br>

Revision as of 15:35, 10 October 2014




Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






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June 2014

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Week 4
Week 5

July 2014

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Week 3
Week 4
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August 2014

Week 1
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Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

June 2014

Week 1
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Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
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August 2014

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September 2014

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