Team:HokkaidoU Japan/Projects/H Stem
From 2014.igem.org
Overview
The weak point of silencing by anti-sense RNA (asRNA) is the instability of RNA. RNA has a shorter half-life than DNA, it is essential to be stabilized for asRNA to repress mRNA sufficiently. Nakashima N and his colleagues solved this problem. They inserted each-complementary sequeces called stem regions into upstream and downstream of as RNA. These regions make asRNA form stem-loop structure, which leads to increase the stability of asRNA dramatically.
We followed in their footsteps. We designed our unique stem sequence called H-stem and producecd a BioBricked vector for asRNA expression so that other iGEMers and researchers can easily use stemed asRNA. Using this vector. Using this vector, you can gain an asRNA-expressing construct just by inserting PCRed target gene into the vector.