Team:HokkaidoU Japan/Notebook/Length/as90 120

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Revision as of 11:42, 12 October 2014

   
    

  

  

Anti-sense 90, 120 Construction

    

      

        

	  

	  
Start
	  
	

	  

	  

PCR & PCR purification

	  
	  

	    

	      

	        

		  
Fri Oct 3, Mon Oct 6

		
	      
	      

	R0040-B0034-E1010-B0015 as RBS XhoI, 90bp as mRFP Nco as RBS XhoI, 120bp as mRFP Nco

	    
	  
	

	  

	  

Digestion

	  
	  

	    

	      

	        

		  
Sat Oct 4

		
	      
	      

Cut as 90, as120, H-stem with NcoI, XhoI(using 10×Cut Smart)

	    
	  
	

	  

	  

Ethanol precipitation

	  
	  

	    

	      

	        

		  
Sat Oct 4

		
	      
	      

as90, as120

	    
	  
	

	  

	  

Gel Extraction

	  
	  

	    

	      

	        

		  
Sat Oct 4

		
	      
	      

H-stem

	    
	  
	

	  

	  

Dephophorylation

	  
	  

	    

	      

	        

		  
Sat Oct 4

		
	      
	      

H-stem

	    
	  
	

	  

	  

Ligation

	  
	  

	    

	      

	        

		  
Sun Oct 5

		
	      
	      

Ligate as90, as120 with H-stem

	    
	  
	

	  

	  

Transformation

	  
	  

	    

	      

	        

		  
Sun Oct 5

		
	      
	      

as90, as120 on H-stem 5µL DNA to DH5α Turbo

	    
	  
	

	  

	  

Colony PCR

	  
	  

	    

	      

	        

		  
Mon Oct 6

		
	      
	      

as90, as120 on H-stem

	    
	  
	

	  

	  

Liquid Culture

	  
	  

	    

	      

	        

		  
Tue Oct 7

		
	      
	      

as90, as120 on H-stem

	    
	  
	

	  

	  

Mini-prep

	  
	  

	    

	      

	        

		  
Wed Oct 8

		
	      
	      

as90, as120 on H-stem

	    
	  
	

	  

	  

Dye terminator

	  
	  

	    

	      

	        

		  
Wed Oct 8

		
	      
	      

as90, as120 on H-stem

	    
	  
	
	

      
Complete!