Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

From 2014.igem.org

(Difference between revisions)
 
Line 252: Line 252:
   <div id="footer-content">
   <div id="footer-content">
<div id="footer-logo">
<div id="footer-logo">
-
<a href="http://igemhokkaidou.com/"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
+
<a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
</div>
</div>
<div id="footer-twitter">
<div id="footer-twitter">

Latest revision as of 15:28, 9 September 2015

Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • Mini-Prep

    R0010-stem-as-stem-B0015 (on pSB1A3)
  • PCR

    R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃
  • Digestion

    pSB1A3 with EcoRI & PstI
    R0010-stem-as-stem-B0015 with EcoRI & PstI
  • Ligation

    Ligate 0010-stem-as-stem-B0015 with pSB1A3
  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)
    5.0µL to DH5alpha;
  • Complete!