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Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction
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Latest revision as of 15:28, 9 September 2015
Notebook
Lab Documents
Stem-Downstream Correction
-
Start
-
Sequencing
R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted! -
PCR
R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9℃ & XhoI_RBS_R Tm:68.2℃ -
Digestion
XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI -
Primer Annealing
stem_downstream_F Tm:℃ & stem_downstream_R -
Ligation
XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3) -
Colony PCR
R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0℃ & 100_up_R Tm:62.6℃ -
Complete!
