"
Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction
From 2014.igem.org
(Difference between revisions)
| Line 90: | Line 90: | ||
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
| - | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 02:49, 18 October 2014
Notebook
Lab Documents
Stem-Downstream Correction
-
Start
-
Sequencing
R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted! -
PCR
R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9℃ & XhoI_RBS_R Tm:68.2℃ -
Digestion
XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI -
Primer Annealing
stem_downstream_F Tm:℃ & stem_downstream_R -
Ligation
XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3) -
Colony PCR
R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0℃ & 100_up_R Tm:62.6℃ -
Complete!
