Team:Heidelberg

From 2014.igem.org

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<h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1>
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<h1 class="very-large-text">Our Application:</h1>
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<p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p>
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<h1 class="dark-grey-text large-text" style="text-align: right;">circular <span class="red-text">heat-stable</span><br>DNA-methyltransferase</h1>
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<p>The problem: DNMT 1, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable.
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<p class="middle-text">Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?</p>
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For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT 1 by circularization.
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<p class="middle-text">See here an ENTIRELY NEW way of performing PCR with a <span class="red-text">heat stable</span> methyltransferase!
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<h1 style="text-align: right; font-size: 3em;" >... and show you the WORLD of <br />
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<h2 class="medium-text align-roght">One more thing.<br />Inteins are capable of mush more!</h2>
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<span class="red-text">post-translational MODIFCATION</span>
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<h1 class="large-text align-right red-text">post-translational MODIFICATIONS</h1>
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<p>The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.</p>
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<p class="align-right normal-medium-text">We created an INTEIN TOOLBOX so you can easily modify your protein in a standardized way.</p>
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<p>Our toolbox contains several tools which are PLACEHOLDER. Here you can find out more about our Toolbox.</p>
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<p class="align-right normal-medium-text">Take a look at our <a href="/Team:Heidelberg/Parts/RFC">RFC</a></p>
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<p>In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.</p>
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<p class="align-right normal-medium-text">Explore all our tools!</p>
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Revision as of 15:53, 15 October 2014

iGEM Logo
Ring of fire Image

iGEM TEAM HEIDELBERG 2014

THE RING
OF FIRE

Click here to view our abstract.
Scroll down to EXPLORE our project.

Nature has made many curious inventions. One of these are

CIRCULAR PROTEINS

which are unconventional peptides that neither have a beginning, nor an ending


These proteins are extremely resistant against high temperatures, pH and proteases.

We established protein circularization as a new
powerful tool for Synthetic Biology and set the foundations
to render any protein heat stable.

Wondering how we circularize?

Let us introduce you to the next generation of bioengineering...

come DISCOVER the MECHANISM of SPLIT INTEINS



Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined

– an effective mechanism to circularize proteins.

Placeholder

But messing with protein structure can be disastrous,
you can only win with good MODELING.



Find out the EXCITING THEORY
behind RIGID LINKERS and their ANGLES


We developed CRAUT,
a comprehensive software which identifies
the optimal path to connect a protein’s termini
preserving structure and function.

Look at our extensive wet-lab SCREENING to improve and calibrate our software using lambda lysozyme!

As calculations on protein structures are costly
we involved the rest of the world with

iGEM@home

Empowering science!


NEW PLATFORM for distributed computing.

Volunteers provide the idle calacity of their home computers.

And, with an established user base with more than 1000 computers, we effectively bring synthetic biology to society in a new way

Our Application:

circular heat-stable
DNA-methyltransferase

Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?

See here an ENTIRELY NEW way of performing PCR with a heat stable methyltransferase!

PCR 2.0

One more thing.
Inteins are capable of mush more!

We show you the WORLD of

post-translational MODIFICATIONS

We created an INTEIN TOOLBOX so you can easily modify your protein in a standardized way.

Take a look at our RFC

Explore all our tools!

circular heat-stable
Xylanase

Xylanase is an important enzyme for the pulp and paper industry.

Bla bla

In future Xylanase could be used for the production of biofuel.

INDUSTRY

Who are we?

We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.

For our project we got great feedback and support from our supervisors.

Take a look at our Teampage!

Thank you!

We want thank all people who helped us and supported our work in the lab.

Retrieved from "http://2014.igem.org/Team:Heidelberg"