From 2014.igem.org

1. Today paper is used in quantity every day and will be waste after use. In Japan, for example, they send new year's card to relations, friends and acquaintances every year. So some cards are thrown away when it is written incorrectly, stained or sent from disagreeable person. It is a sheer waste to throw away many cards, so it is great idea that we transform card into MOCHI, tranditional Japanese food made from rice and composed of amylopectin. Paper contains cellulose, and both amylopectin and cellulose are polyglucose. Why we can't the transformation?

2. In vivo, proteins are synthesized in transcription and translation. Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns which don't code for proteins and joining in exons which code for ones. It occurs after transcription. Splicing is catalyzed by several base sequences of the ends of introns as a ribozyme, being subjected to nucleophilic attack from introns to exons. So we will introduce the plasmids which place the sequence of the end of introns as a splicing ribozyme on the end of a gene coding for proteins into E.coli, and cyclize mRNA for synthesis of long-chain, massive proteins.

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