Team:Gifu
From 2014.igem.org
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+ | <tr> | ||
+ | <td><h2>Theme of our project</h2></td> | ||
+ | </tr> | ||
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+ | <ol type="1"> | ||
+ | <li> | ||
+ | <p>Today paper is used in quantity every day and will be waste after use. | ||
+ | In Japan, for example, they send new year's card to relations, friends and acquaintances every year. | ||
+ | So some cards are thrown away when it is written incorrectly, stained or sent from disagreeable person. | ||
+ | It is a sheer waste to throw away many cards, so it is great idea that we transform card into MOCHI, tranditional Japanese food made from rice and composed of amylopectin. | ||
+ | Paper contains cellulose, and both amylopectin and cellulose are polyglucose. | ||
+ | Why we can't the transformation? | ||
+ | </p> | ||
+ | </li> | ||
+ | <li> | ||
+ | <p>In vivo, proteins are synthesized in transcription and translation. | ||
+ | Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. | ||
+ | In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency. | ||
+ | That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. | ||
+ | To cyclize mRNA, we can use a splicing mechanism of T4 phage. | ||
+ | Splicing is a mechanism removing circular introns which don't code for proteins and joining in exons which code for ones. | ||
+ | It occurs after transcription. | ||
+ | Splicing is catalyzed by several base sequences of the ends of introns as a ribozyme, being subjected to nucleophilic attack from introns to exons. | ||
+ | So we will introduce the plasmids which place the sequence of the end of introns as a splicing ribozyme on the end of a gene coding for proteins into E.coli, and cyclize mRNA for | ||
+ | synthesis of long-chain, massive proteins. | ||
+ | </p> | ||
+ | </li> | ||
+ | </ol> | ||
</table> | </table> |
Revision as of 07:45, 14 August 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Theme of our project |