Team:Gifu

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<h1 style="color:#ccffcc">WELCOME TO iGEM 2014! </h1>
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<p style="color:#330000">Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Gifu&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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  <td><h2>Theme of our project</h2></td>
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<ol type="1">
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  <li>
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    <p>Today paper is used in quantity every day and will be waste after use.
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    In Japan, for example, they send new year's card to relations, friends and acquaintances every year.
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    So some cards are thrown away when it is written incorrectly, stained or sent from disagreeable person.
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    It is a sheer waste to throw away many cards, so it is great idea that we transform card into MOCHI, tranditional Japanese food made from rice and composed of amylopectin.
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    Paper contains cellulose, and both amylopectin and cellulose are polyglucose.
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    Why we can't the transformation?
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    </p>
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  </li>
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  <li>
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    <p>In vivo, proteins are synthesized in transcription and translation.
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    Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA.
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    In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency.
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    That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon.
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    To cyclize mRNA, we can use a splicing mechanism of T4 phage.
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    Splicing is a mechanism removing circular introns which don't code for proteins and joining in exons which code for ones.
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    It occurs after transcription.
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    Splicing is catalyzed by several base sequences of the ends of introns as a ribozyme, being subjected to nucleophilic attack from introns to exons.
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    So we will introduce the plasmids which place the sequence of the end of introns as a splicing ribozyme on the end of a gene coding for proteins into E.coli, and cyclize mRNA for
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    synthesis of long-chain, massive proteins.
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    </p>
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Latest revision as of 07:21, 5 September 2015

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