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Team:Freiburg/Content/Results/Vector
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| - | + | <figure> | |
| + | <img src="https://static.igem.org/mediawiki/2014/9/9d/Freiburg2014-09-10_FACS-specificity.png"> | ||
| + | <figcaption> | ||
| + | <p class="header">Fig.X: FACS analysis of different cell lines incubated with the CAT-1 specific viral vector</p> | ||
| + | <p class="desc">Left: Different cell lines not incubated with the vector as negative control, Middle: different cell lines infected with MuLV IRES EGFP (50% in completed growth medium), Right: cells | ||
| + | transfected with the mouse specific receptor CAT-1 (rz006) were infected with the viral vector. The infection efficiency is directly correlated with transfection | ||
| + | efficiency of the receptor into the different kind of cells.</p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
| + | </section> | ||
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<h2>1.2 Optimization of transduction</h2> | <h2>1.2 Optimization of transduction</h2> | ||
<h2>1.3 Expression of different viral constructs</h2> | <h2>1.3 Expression of different viral constructs</h2> | ||
Revision as of 22:37, 1 October 2014
1 The Vector
1.1 Specificity of MuLV
An important aspect for the function of our system as well as for its safety is the specificity of the vector regarding infection of different kind of cells. The vector deriving from the murine leukemia virus is specific for cells carrying the mouse specific CAT-1. Cells that do not have this specific receptor are not targeted by the vector. In order to test the specificity of the system, different kind of cells were incubated with the vector..
Fig.X: Scheme for testing the specificity of the vector.
In order to test the specificity of the viral vector different cells were incubated with the vector for four hours. Cells not containing the mouse specific receptor CAT-1 are not capable for infection. As control mouse fibroblasts (NIH3t3) expressing CAT-1 were used.
Fig.X: FACS analysis of different cell lines incubated with the CAT-1 specific viral vector
Left: Different cell lines not incubated with the vector as negative control, Middle: different cell lines infected with MuLV IRES EGFP (50% in completed growth medium), Right: cells transfected with the mouse specific receptor CAT-1 (rz006) were infected with the viral vector. The infection efficiency is directly correlated with transfection efficiency of the receptor into the different kind of cells.
