Team:Freiburg/Content/Results/BioBrick

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The AcCELLerator

Bio Bricks

BBa_K1470000: mCat-1

mCat-1 is the recetor we introduced to human cells that they can be infected by the MuLV

BBa_K1470001: Puromycin N-acetyl-transferase

Puromycin N-acetyl-transferase

BBa_K1470002: Galactose-induced gene 4 (Gal4BD)

Galactose-induced gene 4 (Gal4BD)

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)

BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)

Secreted Alkaline Phosphatase (SEAP)

BBa_K1470005: engineered PDZ domain (ePDZ)

engineered PDZ domain (ePDZ)

BBa_K1470006: The Viral Vector (pMIG)

The Viral Vector (pMIG)

BBa_K1470007: Cas9-Nickase

Cas9-Nickase

Plasmids

mCat-1 Plasmids

Constitutive Expression

p14rz_003

- bla

- blaII

p14rz_003: pcDNA_SLC7A1_GFP

p14rz_004

- bla

- blaII

Freiburg2014_p14rz_004_pcDNA_SLC7A1_HA.png

p14rz_005

- bla

- blaII

Freiburg2014_p14rz_005_pcDNA_SLC7A1_mCherry.png

p14rz_006

- bla

- blaII

Freiburg2014_p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry.png

p14rz_007

- bla

- blaII

Freiburg2014_p14rz_007_pcDNA_SLC7A1_HA_mCherry.png

Red Light induced

p14rz_010

Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png

p14rz_011

Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png

Blue Light induced

p14rz_008

Freiburg2014_p14rz_008_Gal4_SLC7A1.png

p14rz_009

Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png

Light System

Red Light

Blue Light

UV Light

p14ls_001

p14ls_001_COP1-VP16_IRES_TetR-UVR8

p14ls_002

Freiburg2014_p14ls_002_Gal4_mCherry

Viral Vector Plasmids

image/svg+xml amp R pUC ori 3´LTR MCS psi_plus_pack 5´LTR PstI (1,463) NotI (1,435) EcoRI (1,426), 2 0 0 4 0 0 6 0 0 8 0 0 1 , 0 0 0 1 , 2 0 0 1 , 4 0 0 1 , 6 0 0 1 , 8 0 0 2 , 0 0 0 2 , 2 0 0 2 , 4 0 0 2 , 6 0 0 2 , 8 0 0 3 , 0 0 0 3 , 2 0 0 3 , 4 0 0 3 , 6 0 0 3 , 8 0 0 4 , 0 0 0 4 , 2 0 0 4 , 4 0 0 4 , 6 0 0 4 , 8 0 0 4 , 9 4 0 BBa_K1470006 (pMIG) 4,940 bp

Reporter Proteins