2 6W of HEK293 cells were transfected with rz006 (receptor labled with mCherry and HA-tag). After each round of splitting cells were analysed by Western blot. Therefore each round one well was splitted 1:2 on two new wells and one well was lysed by Ripa lysis. Afterwards, each lysed sample was frozen in -20°C until use.

2 6W of HEK293 cells were transfected with rz006 (receptor labled with mCherry and HA-tag). After eaasdasdells and one well was lysed by Ripa lysis. Afterwards, each lysed sample was frozen in -20°C until use.