Team:Freiburg/Content/Notebook

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ID Template Primer 1 Primer 2 Size[kbp]  
PCR 1 pKM292 o14zl_039 o14zl_040 1,3 p14zl_010_pLXV_Gal4BD_LOV_puro
PCR 2 p14zl_003 o14zl_041 o14zl_042 0,66 p14zl_010_pLXV_Gal4BD_LOV_puro
PCR 3 pQXIN SLC7A1 o14rz_016 o14rz_017 1,87 p14rz_003_pcDNA_SLC71A_GFP
PCR 4 pQXIN SLC7A1 o14rz_018 o14rz_019 1,87 p14rz_005_pcDNA_SLC7A1_mCherry
PCR 5 pKM078 o14rz_020 o14rz_021 0,78 p14rz_005_pcDNA_SLC7A1_mCherry
PCR 6 p14rz_002 o14rz_022 o14rz_023 1,93 p14rz_004_pcDNA_SLC7A1_HA
PCR 7 pKM084 o14_ls_013 o14_ls_014 3,9 p14ls_002_Gal4_mCherry
PCR 8 pKM078 o14_ls_015 o14_ls_016 0,78 p14ls_002_Gal4_mCherry
PCR 9 pQXIN SLC7A1 o14_ls_017 o14_ls_018 1,93 p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry
PCR 10 pBK001 o14_ls_019 o14_ls_020 0,87 p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry
PCR 11 pKM022 o14_ls_005 o14_ls_006 2,8 p14ls_001_COP1-VP16_TetR_UVR8
PCR 12 Digest 7 o14_ls_008 o14_ls_009 1,4 p14ls_001_COP1-VP16_TetR_UVR8
PCR 13 UVR8 (Bba_1150029) o14_ls_010 o14_ls_011 1,2 p14ls_001_COP1-VP16_TetR_UVR8
PCR 14 psi_pack_synthese o14vv_031 o14vv_032 0,64 pMIG_Standardisierung (Synthese rein)
PCR 15 p14vv_003_backbone o14vv_033 o14vv_034 3,4 pMIG_Standardisierung (XbaI raus)
PCR 16 COP1_VP16(Patrick) o14_ls_007 o14_ls_012 1,5 p14ls_001_COP1-VP16_TetR_UVR8
PCR 17 pBK001 o14_rz_028 o14_rz_029 0,84 p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry
PCR 18 pQXIN SLC7A1 o14_rz_026 o14_rz_027 1,9 p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry
PCR 19 pKM297 o14zl_035 o14zl_036 1,5 p14zl_011_pLXV_ePDZ_VP16_mCherry
PCR 20 pKM078 o14zl_037 o14zl_043 0,8 p14zl_011_pLXV_ePDZ_VP16_mCherry
PCR 21 pCDNA o14rz_024 o14rz_025 5,4 p14rz_004_pcDNA_SLC7A1_HA_2
PCR 22 pQXIN SLC7A1 o14rz_030 o14rz_031 1,9 p14rz_004_pcDNA_SLC7A1_HA_2
PCR 23 pQXIN SLC7A1 o14rz_032 o14rz_033 1,9 p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 24 pBK001 o14rz_034 o14rz_035 0,85 p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 25 p14zl_003 014zl_010 014zl_011 5,4 p14zl_004_pMIG_Phyb_VP16_puro_TetR_PIF6
PCR 26 pKM006 o14vv_037 o14vv_038 1,6 p14vv_006_pMIG_CMV_SEAP
PCR 27 pKM006 o14vv_037 o14vv_039 1,6 p14vv_007_pMIG_SEAP
PCR 28 pLXV o14zl_044_pLXV_rv_neu o14zl_030 6,3 p14zl_008_pLXV_TetO13_CMVmin_SLC7A1
PCR 29 p14rz_006 o14zl_048 o14zl_049 2,7 p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 30 p14vv_003_backbone o14zl_050 o14zl_051 4,9 p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 31 lenti-CRISPR o14zl_020 o14zl_021 0,7 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 32 pKM297 o14zl_022 o14zl_023 1 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 33 pKM292 o14zl_024 o14zl_025 1,4 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 34 pKM022 o14zl_026 o14zl_027 0,6 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 35 p14rz_006 o14rz_033 o14rz_036 2 p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry
PCR 36 pGEMHE-XfA4-mCherry o14rz_035 o14rz_037 0,8 p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry
PCR 37 p14rz_006 o14rz_026 o14rz_036 2 p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry
PCR 38 pBK001 o14rz_029 o14rz_037 0,8 p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry
PCR 39 pKM084 o14rz_040 o14rz_041 3,9 p14rz_009_Gal4_SLC7A1-HA
PCR 40 p14rz_002 o14rz_039 o14rz_038 2 p14rz_009_Gal4_SLC7A1-HA
PCR 41 pMIG o14rz_042 o14rz_043 1,4 p14rz_012_pcDNA_SLC7A1_IRES_GFP
PCR 42 pQXIN SLC7A1 o14rz_044 o14rz_045 2 p14rz_012_pcDNA_SLC7A1_IRES_GFP
PCR 43 mTAG_BFP2 o14vv_044 o14vv_045 0,8 p14vv_008_pMIG_mTAG_BFP2
PCR 44 mKO o14vv_042 o14vv_043 0,8 p14vv_009_pMIG_mKO
PCR 45 mKate o14vv_046 o14vv_047 0,8 p14vv_010_pMIG_mKate
PCR 46 pQXIN SLC7A1 o14vv_050 o14vv_051 2 p14vv_012_pMIG_SLC7A1_IRES_GFP
PCR 47 p14zl_003 o14vv_054 o14vv_055 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 48 p14zl_003 o14vv_052 o14vv_053 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 49 pMIG o14vv_056 o14vv_057 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 50 pMIG o14vv_056 o14vv_059 0,8 p14vv_014_pMIG_eGFP_p2a_puro
PCR 51 p14zl_003 o14vv_055 o14vv_058 0,8 p14vv_014_pMIG_eGFP_p2a_puro
PCR 52 p14zl_003 o14zl_052 o14zl_053 2,4 p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6
PCR 53 p14zl_003 o14zl_054 o14zl_055 2,4 p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6
PCR 54 p14ls_001 o14zl_056 o14zl_057 4 p14zl_015_pMIG_COP1-VP16_TetR_UVR8
PCR 55 p14rz_008_Gal4_SLC7A1 p14zl_061 p14zl_062 0,27 p14zl_018_pLXV_Gal4_GFP_WPRE
PCR 56 pEGFP-C1 p14zl_063 p14zl_064 0,83 p14zl_018_pLXV_Gal4_GFP_WPRE
PCR 57 pMIG-venus p14zl_058 p14zl_059 1,37 p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE
PCR 58 p14rz_008_Gal4_SLC7A1 p14zl_061 p14zl_065 2,13 p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE
PCR 59 pMIG-dsRED p14zl_069 p14zl_068 1,34 p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED
PCR 60 pKM292 p14zl_066 p14zl_067 1,4 p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED
PCR 61 pMIG-Venus p14zl_059 p14zl_076 0,61 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 62 pGEMHE-XfA4-mKate o14zl_074 p14zl_075 0,78 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 63 p14rz_008 p14zl_061 p14zl_065 2,1 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 64 pMIG-CFP p14zl_059 p14zl_058 1,37 p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE
PCR 65 pKM297 o14zl_035 p14zl_060 1,5 p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE
PCR 66 p14rz_008_Gal4_SLC7A1 p14zl_070 p14zl_065 2,1 p14zl_022_pMIG_Gal4_SLC7A1_venus
PCR 67 pMIG-Venus p14zl_059 p14zl_073 1,37 p14zl_022_pMIG_Gal4_SLC7A1_venus
PCR 68 p14rz_008_Gal4_SLC7A1 p14zl_070 p14zl_062 0,26 p14zl_023_pMIG_Gal4_GFP
PCR 69 pEGFP-C1 p14zl_072 p14zl_071 0,83 p14zl_023_pMIG_Gal4_GFP