Team:Freiburg/Content/Notebook

From 2014.igem.org

Revision as of 00:50, 18 October 2014 by Ph123 (Talk | contribs)

p14rz_002

  • PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
  • Digest: pKM006, EcoRI + NotI; PCR-product
  • Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

PCR

Testdigest

p14rz_003

  • PCR 3
  • Digest 3, 4
  • Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

PCR

Digest

Testdigest

p14rz_004

  • PCR 21,22
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

PCR

PCR

Testdigest

p14rz_005

  • PCR 4,5
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

PCR

Digest

Testdigest

p14rz_006

  • PCR 17,18
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

PCR

Digest

Testdigest

p14rz_007

  • PCR 37,38
  • Digest 21
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

PCR

Digest

Testdigest

p14rz_008

  • PCR blue light receptor; Template: pQXCIN-SLC7A1
  • Digest pKM006 EcoRI, NotI
  • Testdigest with SmaI in Cutsmart, expected bands: 5,7

PCR

Testdigest

p14rz_009

  • PCR 39,40
  • Digest 5
  • Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

Digest

Testdigest

p14rz_010

  • PCR 23,24
  • Digest 12
  • Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

PCR

PCR

Testdigest

p14ls_003

  • PCR 7,9,10
  • Digest -
  • Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

PCR

Digest

Testdigest

p14vv_001

  • PCR Primers vv003,vv004; Template: pMIG
  • Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
  • Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

Digest

Digest

Testdigest

p14vv_002

  • PCR -
  • Digest 8,11
  • Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

PCR

Digest

Testdigest

p14vv_003

  • PCR pMIG with primer vv003+vv004-
  • Digest xx
  • Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

PCR

Testdigest

p14vv_006

  • PCR 26
  • Digest 13
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

PCR

Testdigest

p14vv_007

  • PCR 27
  • Digest 14
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

PCR

Testdigest

p14vv_008

  • PCR 43
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

PCR

Digest

Testdigest

p14vv_009

  • PCR 44
  • Digest 22
  • Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

PCR

Digest

Testdigest

p14vv_010

  • PCR 45
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

PCR

Digest

Testdigest

p14vv_012

  • PCR 46
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

PCR

Digest

Testdigest

p14vv_013

  • PCR 47,48,49
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

PCR

Digest

Testdigest

p14vv_014

  • PCR 50,51
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

PCR

Digest

Testdigest

p14zl_010

  • PCR 1,2
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

PCR

Digest

Testdigest

p14zl_011

  • PCR 19,20
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

PCR

Digest

Testdigest

p14zl_016

  • PCR 52,53
  • Digest 22
  • Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

PCR

Digest

Testdigest

p14zl_017

  • PCR 64,65
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

PCR_digest

Testdigest

p14zl_020

  • PCR 59,60
  • Digest 24
  • testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8

PCR

Digest

Testdigest