Team:Freiburg/Content/Notebook

From 2014.igem.org

(Difference between revisions)
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<h2>p14rz_002</h2>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1</li>
 
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<li>Digest: pKM006, EcoRI + NotI; PCR-product</li>
 
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<li>Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7</li>
 
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</ul>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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<img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg">
 
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<p class="header">Testdigest</p>
 
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<h2>p14rz_003</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR 3</li>
 
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<li>Digest 3, 4</li>
 
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<li>Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 
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<figcaption>
 
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<p class="header">Digest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_004</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR 21,22</li>
 
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<li>Digest -</li>
 
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<li>Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
 
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<figcaption>
 
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<p class="header">PCR</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_005</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR 4,5</li>
 
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<li>Digest 5</li>
 
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<li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 
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<figcaption>
 
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<p class="header">Digest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_006</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR 17,18</li>
 
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<li>Digest 5</li>
 
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<li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 
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<figcaption>
 
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<p class="header">Digest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_007</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
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<li>PCR 37,38</li>
 
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<li>Digest 21</li>
 
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<li>Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
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        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 
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<figcaption>
 
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<p class="header">Digest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_008</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
-
<li>PCR blue light receptor; Template: pQXCIN-SLC7A1</li>
 
-
<li>Digest pKM006 EcoRI, NotI</li>
 
-
<li>Testdigest with SmaI in Cutsmart, expected bands: 5,7</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
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<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_009</h2>
 
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<section>
 
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<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
-
<li>PCR 39,40</li>
 
-
<li>Digest 5</li>
 
-
<li>Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
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<div class="row category-row">
 
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      <div class="col-sm-6">
 
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  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 
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<figcaption>
 
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<p class="header">Digest</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14rz_010</h2>
 
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<section>
 
-
<div class="row category-row">
 
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<div class="col-sm-6">
 
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<ul>
 
-
<li>PCR 23,24</li>
 
-
<li>Digest 12</li>
 
-
<li>Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56</li>
 
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</ul>
 
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</div>
 
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<div class="col-sm-6">
 
-
 
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<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
 
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        <figcaption>
 
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<p class="header">PCR</p>
 
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        </figcaption>
 
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    </figure>
 
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</div>
 
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</div>
 
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</section>
 
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<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 
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<figcaption>
 
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<p class="header">PCR</p>
 
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</figcaption>
 
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</figure>
 
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  </div>
 
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<div class="col-sm-6">
 
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  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
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</figcaption>
 
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</figure>
 
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</div>
 
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</div>
 
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</section>
 
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<h2>p14ls_003</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 7,9,10</li>
 
-
<li>Digest -</li>
 
-
<li>Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4</li>
 
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</ul>
 
-
 
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</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
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</div>
 
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</div>
 
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</section>
 
-
 
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<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
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</figure>
 
-
 
 
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-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
 
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<figcaption>
 
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<p class="header">Testdigest</p>
 
-
</figcaption>
 
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</figure>
 
-
 
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</div>
 
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</div>
 
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</section>
 
-
 
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-
<h2>p14vv_001</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR Primers vv003,vv004; Template: pMIG</li>
 
-
<li>Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI</li>
 
-
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
 
-
        <figcaption>
 
-
<p class="header">Digest</p>
 
-
        </figcaption>
 
-
    </figure>
 
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-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
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<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
<h2>p14vv_002</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR -</li>
 
-
<li>Digest 8,11</li>
 
-
<li>Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14vv_003</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR pMIG with primer vv003+vv004-</li>
 
-
<li>Digest xx</li>
 
-
<li>Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14vv_006</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 26</li>
 
-
<li>Digest 13</li>
 
-
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
 
-
<h2>p14vv_007</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 27</li>
 
-
<li>Digest 14</li>
 
-
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<h2>p14vv_008</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 43</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14vv_009</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 44</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
<h2>p14vv_010</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 45</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
<h2>p14vv_012</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 46</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14vv_013</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 47,48,49</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14vv_014</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 50,51</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14zl_010</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 1,2</li>
 
-
<li>Digest 1</li>
 
-
<li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14zl_011</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 19,20</li>
 
-
<li>Digest 1</li>
 
-
<li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14zl_016</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 52,53</li>
 
-
<li>Digest 22</li>
 
-
<li>Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
<h2>p14zl_017</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 64,65</li>
 
-
<li>Digest 24</li>
 
-
<li>testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR_digest</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
 
-
 
-
 
-
<h2>p14zl_020</h2>
 
-
<section>
 
-
<div class="row category-row">
 
-
   
 
-
<div class="col-sm-6">
 
-
<ul>
 
-
<li>PCR 59,60</li>
 
-
<li>Digest 24</li>
 
-
<li>testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8</li>
 
-
</ul>
 
-
 
-
 
-
</div>
 
-
<div class="col-sm-6">
 
-
 
-
<figure>
 
-
        <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
 
-
        <figcaption>
 
-
<p class="header">PCR</p>
 
-
        </figcaption>
 
-
    </figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
-
 
-
<section>
 
-
<div class="row category-row">
 
-
      <div class="col-sm-6">
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 
-
<figcaption>
 
-
<p class="header">Digest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
 
-
 
 
-
 
 
-
  </div>
 
-
<div class="col-sm-6">
 
-
 
-
 
-
  <figure>
 
-
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
 
-
<figcaption>
 
-
<p class="header">Testdigest</p>
 
-
</figcaption>
 
-
</figure>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
</section>
 
 +
<table width="897">
 +
<tbody>
 +
<tr>
 +
<td width="48">ID</td>
 +
<td width="159">Template</td>
 +
<td width="157">Primer 1</td>
 +
<td width="79">Primer 2</td>
 +
<td width="65">Size[kbp]</td>
 +
<td width="389">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 1</td>
 +
<td>pKM292</td>
 +
<td>o14zl_039</td>
 +
<td>o14zl_040</td>
 +
<td>1,3</td>
 +
<td>p14zl_010_pLXV_Gal4BD_LOV_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 2</td>
 +
<td>p14zl_003</td>
 +
<td>o14zl_041</td>
 +
<td>o14zl_042</td>
 +
<td>0,66</td>
 +
<td>p14zl_010_pLXV_Gal4BD_LOV_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 3</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14rz_016</td>
 +
<td>o14rz_017</td>
 +
<td>1,87</td>
 +
<td>p14rz_003_pcDNA_SLC71A_GFP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 4</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14rz_018</td>
 +
<td>o14rz_019</td>
 +
<td>1,87</td>
 +
<td>p14rz_005_pcDNA_SLC7A1_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 5</td>
 +
<td>pKM078</td>
 +
<td>o14rz_020</td>
 +
<td>o14rz_021</td>
 +
<td>0,78</td>
 +
<td>p14rz_005_pcDNA_SLC7A1_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 6</td>
 +
<td>p14rz_002</td>
 +
<td>o14rz_022</td>
 +
<td>o14rz_023</td>
 +
<td>1,93</td>
 +
<td>p14rz_004_pcDNA_SLC7A1_HA</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 7</td>
 +
<td>pKM084</td>
 +
<td>o14_ls_013</td>
 +
<td>o14_ls_014</td>
 +
<td>3,9</td>
 +
<td>p14ls_002_Gal4_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 8</td>
 +
<td>pKM078</td>
 +
<td>o14_ls_015</td>
 +
<td>o14_ls_016</td>
 +
<td>0,78</td>
 +
<td>p14ls_002_Gal4_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 9</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14_ls_017</td>
 +
<td>o14_ls_018</td>
 +
<td>1,93</td>
 +
<td>p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 10</td>
 +
<td>pBK001</td>
 +
<td>o14_ls_019</td>
 +
<td>o14_ls_020</td>
 +
<td>0,87</td>
 +
<td>p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 11</td>
 +
<td>pKM022</td>
 +
<td>o14_ls_005</td>
 +
<td>o14_ls_006</td>
 +
<td>2,8</td>
 +
<td>p14ls_001_COP1-VP16_TetR_UVR8</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 12</td>
 +
<td>Digest 7</td>
 +
<td>o14_ls_008</td>
 +
<td>o14_ls_009</td>
 +
<td>1,4</td>
 +
<td>p14ls_001_COP1-VP16_TetR_UVR8</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 13</td>
 +
<td>UVR8 (Bba_1150029)</td>
 +
<td>o14_ls_010</td>
 +
<td>o14_ls_011</td>
 +
<td>1,2</td>
 +
<td>p14ls_001_COP1-VP16_TetR_UVR8</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 14</td>
 +
<td>psi_pack_synthese</td>
 +
<td>o14vv_031</td>
 +
<td>o14vv_032</td>
 +
<td>0,64</td>
 +
<td>pMIG_Standardisierung (Synthese rein)</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 15</td>
 +
<td>p14vv_003_backbone</td>
 +
<td>o14vv_033</td>
 +
<td>o14vv_034</td>
 +
<td>3,4</td>
 +
<td>pMIG_Standardisierung (XbaI raus)</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 16</td>
 +
<td>COP1_VP16(Patrick)</td>
 +
<td>o14_ls_007</td>
 +
<td>o14_ls_012</td>
 +
<td>1,5</td>
 +
<td>p14ls_001_COP1-VP16_TetR_UVR8</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 17</td>
 +
<td>pBK001</td>
 +
<td>o14_rz_028</td>
 +
<td>o14_rz_029</td>
 +
<td>0,84</td>
 +
<td>p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 18</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14_rz_026</td>
 +
<td>o14_rz_027</td>
 +
<td>1,9</td>
 +
<td>p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 19</td>
 +
<td>pKM297</td>
 +
<td>o14zl_035</td>
 +
<td>o14zl_036</td>
 +
<td>1,5</td>
 +
<td>p14zl_011_pLXV_ePDZ_VP16_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 20</td>
 +
<td>pKM078</td>
 +
<td>o14zl_037</td>
 +
<td>o14zl_043</td>
 +
<td>0,8</td>
 +
<td>p14zl_011_pLXV_ePDZ_VP16_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 21</td>
 +
<td>pCDNA</td>
 +
<td>o14rz_024</td>
 +
<td>o14rz_025</td>
 +
<td>5,4</td>
 +
<td>p14rz_004_pcDNA_SLC7A1_HA_2</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 22</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14rz_030</td>
 +
<td>o14rz_031</td>
 +
<td>1,9</td>
 +
<td>p14rz_004_pcDNA_SLC7A1_HA_2</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 23</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14rz_032</td>
 +
<td>o14rz_033</td>
 +
<td>1,9</td>
 +
<td>p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 24</td>
 +
<td>pBK001</td>
 +
<td>o14rz_034</td>
 +
<td>o14rz_035</td>
 +
<td>0,85</td>
 +
<td>p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 25</td>
 +
<td>p14zl_003</td>
 +
<td>014zl_010</td>
 +
<td>014zl_011</td>
 +
<td>5,4</td>
 +
<td>p14zl_004_pMIG_Phyb_VP16_puro_TetR_PIF6</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 26</td>
 +
<td>pKM006</td>
 +
<td>o14vv_037</td>
 +
<td>o14vv_038</td>
 +
<td>1,6</td>
 +
<td>p14vv_006_pMIG_CMV_SEAP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 27</td>
 +
<td>pKM006</td>
 +
<td>o14vv_037</td>
 +
<td>o14vv_039</td>
 +
<td>1,6</td>
 +
<td>p14vv_007_pMIG_SEAP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 28</td>
 +
<td>pLXV</td>
 +
<td>o14zl_044_pLXV_rv_neu</td>
 +
<td>o14zl_030</td>
 +
<td>6,3</td>
 +
<td>p14zl_008_pLXV_TetO13_CMVmin_SLC7A1</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 29</td>
 +
<td>p14rz_006</td>
 +
<td>o14zl_048</td>
 +
<td>o14zl_049</td>
 +
<td>2,7</td>
 +
<td>p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 30</td>
 +
<td>p14vv_003_backbone</td>
 +
<td>o14zl_050</td>
 +
<td>o14zl_051</td>
 +
<td>4,9</td>
 +
<td>p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 31</td>
 +
<td>lenti-CRISPR</td>
 +
<td>o14zl_020</td>
 +
<td>o14zl_021</td>
 +
<td>0,7</td>
 +
<td>p14zl_007_pLXV_LOV_ePDZ_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 32</td>
 +
<td>pKM297</td>
 +
<td>o14zl_022</td>
 +
<td>o14zl_023</td>
 +
<td>1</td>
 +
<td>p14zl_007_pLXV_LOV_ePDZ_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 33</td>
 +
<td>pKM292</td>
 +
<td>o14zl_024</td>
 +
<td>o14zl_025</td>
 +
<td>1,4</td>
 +
<td>p14zl_007_pLXV_LOV_ePDZ_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 34</td>
 +
<td>pKM022</td>
 +
<td>o14zl_026</td>
 +
<td>o14zl_027</td>
 +
<td>0,6</td>
 +
<td>p14zl_007_pLXV_LOV_ePDZ_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 35</td>
 +
<td>p14rz_006</td>
 +
<td>o14rz_033</td>
 +
<td>o14rz_036</td>
 +
<td>2</td>
 +
<td>p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 36</td>
 +
<td>pGEMHE-XfA4-mCherry</td>
 +
<td>o14rz_035</td>
 +
<td>o14rz_037</td>
 +
<td>0,8</td>
 +
<td>p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 37</td>
 +
<td>p14rz_006</td>
 +
<td>o14rz_026</td>
 +
<td>o14rz_036</td>
 +
<td>2</td>
 +
<td>p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 38</td>
 +
<td>pBK001</td>
 +
<td>o14rz_029</td>
 +
<td>o14rz_037</td>
 +
<td>0,8</td>
 +
<td>p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 39</td>
 +
<td>pKM084</td>
 +
<td>o14rz_040</td>
 +
<td>o14rz_041</td>
 +
<td>3,9</td>
 +
<td>p14rz_009_Gal4_SLC7A1-HA</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 40</td>
 +
<td>p14rz_002</td>
 +
<td>o14rz_039</td>
 +
<td>o14rz_038</td>
 +
<td>2</td>
 +
<td>p14rz_009_Gal4_SLC7A1-HA</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 41</td>
 +
<td>pMIG</td>
 +
<td>o14rz_042</td>
 +
<td>o14rz_043</td>
 +
<td>1,4</td>
 +
<td>p14rz_012_pcDNA_SLC7A1_IRES_GFP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 42</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14rz_044</td>
 +
<td>o14rz_045</td>
 +
<td>2</td>
 +
<td>p14rz_012_pcDNA_SLC7A1_IRES_GFP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 43</td>
 +
<td>mTAG_BFP2</td>
 +
<td>o14vv_044</td>
 +
<td>o14vv_045</td>
 +
<td>0,8</td>
 +
<td>p14vv_008_pMIG_mTAG_BFP2</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 44</td>
 +
<td>mKO</td>
 +
<td>o14vv_042</td>
 +
<td>o14vv_043</td>
 +
<td>0,8</td>
 +
<td>p14vv_009_pMIG_mKO</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 45</td>
 +
<td>mKate</td>
 +
<td>o14vv_046</td>
 +
<td>o14vv_047</td>
 +
<td>0,8</td>
 +
<td>p14vv_010_pMIG_mKate</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 46</td>
 +
<td>pQXIN SLC7A1</td>
 +
<td>o14vv_050</td>
 +
<td>o14vv_051</td>
 +
<td>2</td>
 +
<td>p14vv_012_pMIG_SLC7A1_IRES_GFP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 47</td>
 +
<td>p14zl_003</td>
 +
<td>o14vv_054</td>
 +
<td>o14vv_055</td>
 +
<td>0,7</td>
 +
<td>p14vv_013_pMIG_eGFP_IRES_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 48</td>
 +
<td>p14zl_003</td>
 +
<td>o14vv_052</td>
 +
<td>o14vv_053</td>
 +
<td>0,7</td>
 +
<td>p14vv_013_pMIG_eGFP_IRES_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 49</td>
 +
<td>pMIG</td>
 +
<td>o14vv_056</td>
 +
<td>o14vv_057</td>
 +
<td>0,7</td>
 +
<td>p14vv_013_pMIG_eGFP_IRES_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 50</td>
 +
<td>pMIG</td>
 +
<td>o14vv_056</td>
 +
<td>o14vv_059</td>
 +
<td>0,8</td>
 +
<td>p14vv_014_pMIG_eGFP_p2a_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 51</td>
 +
<td>p14zl_003</td>
 +
<td>o14vv_055</td>
 +
<td>o14vv_058</td>
 +
<td>0,8</td>
 +
<td>p14vv_014_pMIG_eGFP_p2a_puro</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 52</td>
 +
<td>p14zl_003</td>
 +
<td>o14zl_052</td>
 +
<td>o14zl_053</td>
 +
<td>2,4</td>
 +
<td>p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 53</td>
 +
<td>p14zl_003</td>
 +
<td>o14zl_054</td>
 +
<td>o14zl_055</td>
 +
<td>2,4</td>
 +
<td>p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 54</td>
 +
<td>p14ls_001</td>
 +
<td>o14zl_056</td>
 +
<td>o14zl_057</td>
 +
<td>4</td>
 +
<td>p14zl_015_pMIG_COP1-VP16_TetR_UVR8</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 55</td>
 +
<td>p14rz_008_Gal4_SLC7A1</td>
 +
<td>p14zl_061</td>
 +
<td>p14zl_062</td>
 +
<td>0,27</td>
 +
<td>p14zl_018_pLXV_Gal4_GFP_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 56</td>
 +
<td>pEGFP-C1</td>
 +
<td>p14zl_063</td>
 +
<td>p14zl_064</td>
 +
<td>0,83</td>
 +
<td>p14zl_018_pLXV_Gal4_GFP_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 57</td>
 +
<td>pMIG-venus</td>
 +
<td>p14zl_058</td>
 +
<td>p14zl_059</td>
 +
<td>1,37</td>
 +
<td>p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 58</td>
 +
<td>p14rz_008_Gal4_SLC7A1</td>
 +
<td>p14zl_061</td>
 +
<td>p14zl_065</td>
 +
<td>2,13</td>
 +
<td>p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 59</td>
 +
<td>pMIG-dsRED</td>
 +
<td>p14zl_069</td>
 +
<td>p14zl_068</td>
 +
<td>1,34</td>
 +
<td>p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 60</td>
 +
<td>pKM292</td>
 +
<td>p14zl_066</td>
 +
<td>p14zl_067</td>
 +
<td>1,4</td>
 +
<td>p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 61</td>
 +
<td>pMIG-Venus</td>
 +
<td>p14zl_059</td>
 +
<td>p14zl_076</td>
 +
<td>0,61</td>
 +
<td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 62</td>
 +
<td>pGEMHE-XfA4-mKate</td>
 +
<td>o14zl_074</td>
 +
<td>p14zl_075</td>
 +
<td>0,78</td>
 +
<td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 63</td>
 +
<td>p14rz_008</td>
 +
<td>p14zl_061</td>
 +
<td>p14zl_065</td>
 +
<td>2,1</td>
 +
<td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 64</td>
 +
<td>pMIG-CFP</td>
 +
<td>p14zl_059</td>
 +
<td>p14zl_058</td>
 +
<td>1,37</td>
 +
<td>p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 65</td>
 +
<td>pKM297</td>
 +
<td>o14zl_035</td>
 +
<td>p14zl_060</td>
 +
<td>1,5</td>
 +
<td>p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 66</td>
 +
<td>p14rz_008_Gal4_SLC7A1</td>
 +
<td>p14zl_070</td>
 +
<td>p14zl_065</td>
 +
<td>2,1</td>
 +
<td>p14zl_022_pMIG_Gal4_SLC7A1_venus</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 67</td>
 +
<td>pMIG-Venus</td>
 +
<td>p14zl_059</td>
 +
<td>p14zl_073</td>
 +
<td>1,37</td>
 +
<td>p14zl_022_pMIG_Gal4_SLC7A1_venus</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 68</td>
 +
<td>p14rz_008_Gal4_SLC7A1</td>
 +
<td>p14zl_070</td>
 +
<td>p14zl_062</td>
 +
<td>0,26</td>
 +
<td>p14zl_023_pMIG_Gal4_GFP</td>
 +
</tr>
 +
<tr>
 +
<td>PCR 69</td>
 +
<td>pEGFP-C1</td>
 +
<td>p14zl_072</td>
 +
<td>p14zl_071</td>
 +
<td>0,83</td>
 +
<td>p14zl_023_pMIG_Gal4_GFP</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p>&nbsp;</p>
</body>
</body>
</html>
</html>

Revision as of 01:05, 18 October 2014

ID Template Primer 1 Primer 2 Size[kbp]  
PCR 1 pKM292 o14zl_039 o14zl_040 1,3 p14zl_010_pLXV_Gal4BD_LOV_puro
PCR 2 p14zl_003 o14zl_041 o14zl_042 0,66 p14zl_010_pLXV_Gal4BD_LOV_puro
PCR 3 pQXIN SLC7A1 o14rz_016 o14rz_017 1,87 p14rz_003_pcDNA_SLC71A_GFP
PCR 4 pQXIN SLC7A1 o14rz_018 o14rz_019 1,87 p14rz_005_pcDNA_SLC7A1_mCherry
PCR 5 pKM078 o14rz_020 o14rz_021 0,78 p14rz_005_pcDNA_SLC7A1_mCherry
PCR 6 p14rz_002 o14rz_022 o14rz_023 1,93 p14rz_004_pcDNA_SLC7A1_HA
PCR 7 pKM084 o14_ls_013 o14_ls_014 3,9 p14ls_002_Gal4_mCherry
PCR 8 pKM078 o14_ls_015 o14_ls_016 0,78 p14ls_002_Gal4_mCherry
PCR 9 pQXIN SLC7A1 o14_ls_017 o14_ls_018 1,93 p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry
PCR 10 pBK001 o14_ls_019 o14_ls_020 0,87 p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry
PCR 11 pKM022 o14_ls_005 o14_ls_006 2,8 p14ls_001_COP1-VP16_TetR_UVR8
PCR 12 Digest 7 o14_ls_008 o14_ls_009 1,4 p14ls_001_COP1-VP16_TetR_UVR8
PCR 13 UVR8 (Bba_1150029) o14_ls_010 o14_ls_011 1,2 p14ls_001_COP1-VP16_TetR_UVR8
PCR 14 psi_pack_synthese o14vv_031 o14vv_032 0,64 pMIG_Standardisierung (Synthese rein)
PCR 15 p14vv_003_backbone o14vv_033 o14vv_034 3,4 pMIG_Standardisierung (XbaI raus)
PCR 16 COP1_VP16(Patrick) o14_ls_007 o14_ls_012 1,5 p14ls_001_COP1-VP16_TetR_UVR8
PCR 17 pBK001 o14_rz_028 o14_rz_029 0,84 p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry
PCR 18 pQXIN SLC7A1 o14_rz_026 o14_rz_027 1,9 p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry
PCR 19 pKM297 o14zl_035 o14zl_036 1,5 p14zl_011_pLXV_ePDZ_VP16_mCherry
PCR 20 pKM078 o14zl_037 o14zl_043 0,8 p14zl_011_pLXV_ePDZ_VP16_mCherry
PCR 21 pCDNA o14rz_024 o14rz_025 5,4 p14rz_004_pcDNA_SLC7A1_HA_2
PCR 22 pQXIN SLC7A1 o14rz_030 o14rz_031 1,9 p14rz_004_pcDNA_SLC7A1_HA_2
PCR 23 pQXIN SLC7A1 o14rz_032 o14rz_033 1,9 p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 24 pBK001 o14rz_034 o14rz_035 0,85 p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 25 p14zl_003 014zl_010 014zl_011 5,4 p14zl_004_pMIG_Phyb_VP16_puro_TetR_PIF6
PCR 26 pKM006 o14vv_037 o14vv_038 1,6 p14vv_006_pMIG_CMV_SEAP
PCR 27 pKM006 o14vv_037 o14vv_039 1,6 p14vv_007_pMIG_SEAP
PCR 28 pLXV o14zl_044_pLXV_rv_neu o14zl_030 6,3 p14zl_008_pLXV_TetO13_CMVmin_SLC7A1
PCR 29 p14rz_006 o14zl_048 o14zl_049 2,7 p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 30 p14vv_003_backbone o14zl_050 o14zl_051 4,9 p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
PCR 31 lenti-CRISPR o14zl_020 o14zl_021 0,7 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 32 pKM297 o14zl_022 o14zl_023 1 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 33 pKM292 o14zl_024 o14zl_025 1,4 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 34 pKM022 o14zl_026 o14zl_027 0,6 p14zl_007_pLXV_LOV_ePDZ_puro
PCR 35 p14rz_006 o14rz_033 o14rz_036 2 p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry
PCR 36 pGEMHE-XfA4-mCherry o14rz_035 o14rz_037 0,8 p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry
PCR 37 p14rz_006 o14rz_026 o14rz_036 2 p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry
PCR 38 pBK001 o14rz_029 o14rz_037 0,8 p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry
PCR 39 pKM084 o14rz_040 o14rz_041 3,9 p14rz_009_Gal4_SLC7A1-HA
PCR 40 p14rz_002 o14rz_039 o14rz_038 2 p14rz_009_Gal4_SLC7A1-HA
PCR 41 pMIG o14rz_042 o14rz_043 1,4 p14rz_012_pcDNA_SLC7A1_IRES_GFP
PCR 42 pQXIN SLC7A1 o14rz_044 o14rz_045 2 p14rz_012_pcDNA_SLC7A1_IRES_GFP
PCR 43 mTAG_BFP2 o14vv_044 o14vv_045 0,8 p14vv_008_pMIG_mTAG_BFP2
PCR 44 mKO o14vv_042 o14vv_043 0,8 p14vv_009_pMIG_mKO
PCR 45 mKate o14vv_046 o14vv_047 0,8 p14vv_010_pMIG_mKate
PCR 46 pQXIN SLC7A1 o14vv_050 o14vv_051 2 p14vv_012_pMIG_SLC7A1_IRES_GFP
PCR 47 p14zl_003 o14vv_054 o14vv_055 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 48 p14zl_003 o14vv_052 o14vv_053 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 49 pMIG o14vv_056 o14vv_057 0,7 p14vv_013_pMIG_eGFP_IRES_puro
PCR 50 pMIG o14vv_056 o14vv_059 0,8 p14vv_014_pMIG_eGFP_p2a_puro
PCR 51 p14zl_003 o14vv_055 o14vv_058 0,8 p14vv_014_pMIG_eGFP_p2a_puro
PCR 52 p14zl_003 o14zl_052 o14zl_053 2,4 p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6
PCR 53 p14zl_003 o14zl_054 o14zl_055 2,4 p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6
PCR 54 p14ls_001 o14zl_056 o14zl_057 4 p14zl_015_pMIG_COP1-VP16_TetR_UVR8
PCR 55 p14rz_008_Gal4_SLC7A1 p14zl_061 p14zl_062 0,27 p14zl_018_pLXV_Gal4_GFP_WPRE
PCR 56 pEGFP-C1 p14zl_063 p14zl_064 0,83 p14zl_018_pLXV_Gal4_GFP_WPRE
PCR 57 pMIG-venus p14zl_058 p14zl_059 1,37 p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE
PCR 58 p14rz_008_Gal4_SLC7A1 p14zl_061 p14zl_065 2,13 p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE
PCR 59 pMIG-dsRED p14zl_069 p14zl_068 1,34 p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED
PCR 60 pKM292 p14zl_066 p14zl_067 1,4 p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED
PCR 61 pMIG-Venus p14zl_059 p14zl_076 0,61 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 62 pGEMHE-XfA4-mKate o14zl_074 p14zl_075 0,78 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 63 p14rz_008 p14zl_061 p14zl_065 2,1 p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE
PCR 64 pMIG-CFP p14zl_059 p14zl_058 1,37 p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE
PCR 65 pKM297 o14zl_035 p14zl_060 1,5 p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE
PCR 66 p14rz_008_Gal4_SLC7A1 p14zl_070 p14zl_065 2,1 p14zl_022_pMIG_Gal4_SLC7A1_venus
PCR 67 pMIG-Venus p14zl_059 p14zl_073 1,37 p14zl_022_pMIG_Gal4_SLC7A1_venus
PCR 68 p14rz_008_Gal4_SLC7A1 p14zl_070 p14zl_062 0,26 p14zl_023_pMIG_Gal4_GFP
PCR 69 pEGFP-C1 p14zl_072 p14zl_071 0,83 p14zl_023_pMIG_Gal4_GFP