Team:Freiburg/Content/Notebook

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Revision as of 00:07, 18 October 2014

Contents

p14rz_002

<section>

  • PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
  • Digest: pKM006, EcoRI + NotI; PCR-product
  • Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7


<figure> 

       <img src="2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>



<section>

<figure> <img src="2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg"> <figcaption>

Testdigest

</figcaption> </figure>




</section>





p14rz_003

<section>

  • PCR 3
  • Digest 3, 4
  • Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8


<figure> 

       <img src="2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14rz_004

<section>

  • PCR 21,22
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2


<figure> 

       <img src="2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg"> <figcaption>

PCR

</figcaption> </figure>



<figure> <img src="2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>




p14rz_005

<section>

  • PCR 4,5
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3


<figure> 

       <img src="2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg"> <figcaption>

Digest

</figcaption> </figure>



</section>



p14rz_006

<section>

  • PCR 17,18
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4


<figure> 

       <img src="2014Freiburg_140811_PCR1%2C17%2C18.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14rz_007

<section>

  • PCR 37,38
  • Digest 21
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14rz_008

<section>

  • PCR blue light receptor; Template: pQXCIN-SLC7A1
  • Digest pKM006 EcoRI, NotI
  • Testdigest with SmaI in Cutsmart, expected bands: 5,7


<figure> 

       <img src="2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg"> <figcaption>

Testdigest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14rz_009

<section>

  • PCR 39,40
  • Digest 5
  • Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14rz_010

<section>

  • PCR 23,24
  • Digest 12
  • Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56


<figure> 

       <img src="2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg"> <figcaption>

PCR

</figcaption> </figure>



<figure> <img src="2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14ls_002

<section>

  • PCR 7,8
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;0,56


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14ls_003

<section>

  • PCR 7,9,10
  • Digest -
  • Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4


<figure> 

       <img src="2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_001

<section>

  • PCR Primers vv003,vv004; Template: pMIG
  • Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
  • Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6


<figure> 

       <img src="2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
       <figcaption>

Digest

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14vv_002

<section>

  • PCR -
  • Digest 8,11
  • Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4


<figure> 

       <img src="2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140822_Digest8%2C_11%2C_10.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_003

<section>

  • PCR pMIG with primer vv003+vv004-
  • Digest xx
  • Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5


<figure> 

       <img src="2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_006

<section>

  • PCR 26
  • Digest 13
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6


<figure> 

       <img src="2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14vv_007

<section>

  • PCR 27
  • Digest 14
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66


<figure> 

       <img src="2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_9_10_2014_2_13_33_PM_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>




p14vv_008

<section>

  • PCR 43
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_009

<section>

  • PCR 44
  • Digest 22
  • Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_9_10_2014_2_13_33_PM_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14vv_010

<section>

  • PCR 45
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_9_10_2014_2_13_33_PM_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14vv_012

<section>

  • PCR 46
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_013

<section>

  • PCR 47,48,49
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14vv_014

<section>

  • PCR 50,51
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14zl_003

<section>

  • PCR xx
  • Digest xx
  • Testdigest with NruI+MluI in Cutsmart, expected bands:  4,9;3,3;2,7;0,7
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,7;4,1;2,8


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>




p14zl_010

<section>

  • PCR 1,2
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1


<figure> 

       <img src="2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_011

<section>

  • PCR 19,20
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43


<figure> 

       <img src="2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_016

<section>

  • PCR 52,53
  • Digest 22
  • Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_017

<section>

  • PCR 64,65
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9


<figure> 

       <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
       <figcaption>

PCR_digest

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

PCR

</figcaption> </figure>



<figure> <img src="2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_018

<section>

  • PCR 55,56
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 4,7;2,5


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_019

<section>

  • PCR 57,58
  • Digest 24
  • testdigest with PstI in Cutsmart, expected bands: 5,8;2,6;1,1;0,2


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>


p14zl_020

<section>

  • PCR 59,60
  • Digest 24
  • testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8


<figure> 

       <img src="2014Freiburg_9_10_2014_2_10_51_PM.jpg">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src="2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg"> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src="2014Freiburg_9_10_2014_2_10_51_PM.jpg"> <figcaption>

Testdigest

</figcaption> </figure>


</section>



p14zl_023

<section>

  • PCR 68,69
  • Digest 22
  • testdigest with EcoRV in Cutsmart, expected bands: 3,4;2,5


<figure> 

       <img src="">
       <figcaption>

PCR

       </figcaption>
   </figure>


</section>

<section>

<figure> <img src=""> <figcaption>

Digest

</figcaption> </figure>



<figure> <img src=""> <figcaption>

Testdigest

</figcaption> </figure>


</section>

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