Team:Freiburg/Content/Notebook

From 2014.igem.org

(Difference between revisions)
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</head>
</head>
<body>
<body>
-
<section id="Notebook-Overview">
+
 
-
<h1>Labjournal and Methods</h1>
+
 
-
    <div class="row category-row">
+
<h2>p14rz_002</h2>
-
<div class="col-sm-6">
+
<section>
-
<a href="https://2014.igem.org/Team:Freiburg/Notebook/Labjournal">Labjournal</a>
+
<div class="row category-row">
-
</div>
+
   
-
<div class="col-sm-6">
+
<div class="col-sm-6">
-
<a href="https://2014.igem.org/Team:Freiburg/Notebook/Methods">Methods</a>
+
<ul>
-
</div>
+
<li>PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1</li>
 +
<li>Digest: pKM006, EcoRI + NotI; PCR-product</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7</li>
 +
</ul>
 +
 +
</div>
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<h2>p14rz_003</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 3</li>
 +
<li>Digest 3, 4</li>
 +
<li>Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14rz_004</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 21,22</li>
 +
<li>Digest -</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
 +
<figcaption>
 +
<p class="header">PCR</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
 +
<h2>p14rz_005</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 4,5</li>
 +
<li>Digest 5</li>
 +
<li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<h2>p14rz_006</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 17,18</li>
 +
<li>Digest 5</li>
 +
<li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14rz_007</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 37,38</li>
 +
<li>Digest 21</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14rz_008</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR blue light receptor; Template: pQXCIN-SLC7A1</li>
 +
<li>Digest pKM006 EcoRI, NotI</li>
 +
<li>Testdigest with SmaI in Cutsmart, expected bands: 5,7</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14rz_009</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 39,40</li>
 +
<li>Digest 5</li>
 +
<li>Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14rz_010</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 23,24</li>
 +
<li>Digest 12</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 +
<figcaption>
 +
<p class="header">PCR</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
<h2>p14ls_002</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 7,8</li>
 +
<li>Digest -</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands: 4,1;0,56</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14ls_003</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 7,9,10</li>
 +
<li>Digest -</li>
 +
<li>Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_001</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR Primers vv003,vv004; Template: pMIG</li>
 +
<li>Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
 +
        <figcaption>
 +
<p class="header">Digest</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<h2>p14vv_002</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR -</li>
 +
<li>Digest 8,11</li>
 +
<li>Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_003</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR pMIG with primer vv003+vv004-</li>
 +
<li>Digest xx</li>
 +
<li>Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_006</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 26</li>
 +
<li>Digest 13</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14vv_007</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 27</li>
 +
<li>Digest 14</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
 +
<h2>p14vv_008</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 43</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_009</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 44</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<h2>p14vv_010</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 45</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<h2>p14vv_012</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 46</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_013</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 47,48,49</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14vv_014</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 50,51</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
<h2>p14zl_003</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR xx</li>
 +
<li>Digest xx</li>
 +
<li>Testdigest with NruI+MluI in Cutsmart, expected bands:&nbsp; 4,9;3,3;2,7;0,7</li>
 +
<li>Testdigest with NgoMIV in Cutsmart, expected bands: 4,7;4,1;2,8</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
 +
 +
 +
<h2>p14zl_010</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 1,2</li>
 +
<li>Digest 1</li>
 +
<li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_011</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 19,20</li>
 +
<li>Digest 1</li>
 +
<li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_016</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 52,53</li>
 +
<li>Digest 22</li>
 +
<li>Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_017</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 64,65</li>
 +
<li>Digest 24</li>
 +
<li>testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 +
        <figcaption>
 +
<p class="header">PCR_digest</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">PCR</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_018</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 55,56</li>
 +
<li>Digest 24</li>
 +
<li>testdigest with NgoMIV in Cutsmart, expected bands: 4,7;2,5</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_019</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 57,58</li>
 +
<li>Digest 24</li>
 +
<li>testdigest with <strong>PstI </strong>in Cutsmart, expected bands: 5,8;2,6;1,1;0,2</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<h2>p14zl_020</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 59,60</li>
 +
<li>Digest 24</li>
 +
<li>testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<h2>p14zl_023</h2>
 +
<section>
 +
<div class="row category-row">
 +
   
 +
<div class="col-sm-6">
 +
<ul>
 +
<li>PCR 68,69</li>
 +
<li>Digest 22</li>
 +
<li>testdigest with EcoRV in Cutsmart, expected bands: 3,4;2,5</li>
 +
</ul>
 +
 +
 +
</div>
 +
<div class="col-sm-6">
 +
 +
<figure>
 +
        <img src="">
 +
        <figcaption>
 +
<p class="header">PCR</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
 +
</div>
 +
</div>
 +
</section>
 +
 +
<section>
 +
<div class="row category-row">
 +
      <div class="col-sm-6">
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Digest</p>
 +
</figcaption>
 +
</figure>
 +
 
 +
 
 +
 
 +
  </div>
 +
<div class="col-sm-6">
 +
 +
 +
  <figure>
 +
<img src="">
 +
<figcaption>
 +
<p class="header">Testdigest</p>
 +
</figcaption>
 +
</figure>
 +
 +
 +
</div>
 +
</div>
</section>
</section>
 +
 +
 +
 +
</body>
</body>
</html>
</html>

Revision as of 23:56, 17 October 2014

The AcCELLerator

p14rz_002

  • PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
  • Digest: pKM006, EcoRI + NotI; PCR-product
  • Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

PCR

Testdigest

p14rz_003

  • PCR 3
  • Digest 3, 4
  • Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

PCR

Digest

Testdigest

p14rz_004

  • PCR 21,22
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

PCR

PCR

Testdigest

p14rz_005

  • PCR 4,5
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

PCR

Digest

Testdigest

p14rz_006

  • PCR 17,18
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

PCR

Digest

Testdigest

p14rz_007

  • PCR 37,38
  • Digest 21
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

PCR

Digest

Testdigest

p14rz_008

  • PCR blue light receptor; Template: pQXCIN-SLC7A1
  • Digest pKM006 EcoRI, NotI
  • Testdigest with SmaI in Cutsmart, expected bands: 5,7

PCR

Testdigest

Testdigest

p14rz_009

  • PCR 39,40
  • Digest 5
  • Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

PCR

Digest

Testdigest

p14rz_010

  • PCR 23,24
  • Digest 12
  • Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

PCR

PCR

Testdigest

p14ls_002

  • PCR 7,8
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;0,56

PCR

Digest

Testdigest

p14ls_003

  • PCR 7,9,10
  • Digest -
  • Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

PCR

Digest

Testdigest

p14vv_001

  • PCR Primers vv003,vv004; Template: pMIG
  • Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
  • Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

Digest

Digest

Testdigest

p14vv_002

  • PCR -
  • Digest 8,11
  • Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

PCR

Digest

Testdigest

p14vv_003

  • PCR pMIG with primer vv003+vv004-
  • Digest xx
  • Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

PCR

Digest

Testdigest

p14vv_006

  • PCR 26
  • Digest 13
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

PCR

Digest

Testdigest

p14vv_007

  • PCR 27
  • Digest 14
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

PCR

Digest

Testdigest

p14vv_008

  • PCR 43
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

PCR

Digest

Testdigest

p14vv_009

  • PCR 44
  • Digest 22
  • Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

PCR

Digest

Testdigest

p14vv_010

  • PCR 45
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

PCR

Digest

Testdigest

p14vv_012

  • PCR 46
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

PCR

Digest

Testdigest

p14vv_013

  • PCR 47,48,49
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

PCR

Digest

Testdigest

p14vv_014

  • PCR 50,51
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

PCR

Digest

Testdigest

p14zl_003

  • PCR xx
  • Digest xx
  • Testdigest with NruI+MluI in Cutsmart, expected bands:  4,9;3,3;2,7;0,7
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,7;4,1;2,8

PCR

Digest

Testdigest

p14zl_010

  • PCR 1,2
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

PCR

Digest

Testdigest

p14zl_011

  • PCR 19,20
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

PCR

Digest

Testdigest

p14zl_016

  • PCR 52,53
  • Digest 22
  • Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

PCR

Digest

Testdigest

p14zl_017

  • PCR 64,65
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

PCR_digest

PCR

Testdigest

p14zl_018

  • PCR 55,56
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 4,7;2,5

PCR

Digest

Testdigest

p14zl_019

  • PCR 57,58
  • Digest 24
  • testdigest with PstI in Cutsmart, expected bands: 5,8;2,6;1,1;0,2

PCR

Digest

Testdigest

p14zl_020

  • PCR 59,60
  • Digest 24
  • testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8

PCR

Digest

Testdigest

p14zl_023

  • PCR 68,69
  • Digest 22
  • testdigest with EcoRV in Cutsmart, expected bands: 3,4;2,5

PCR

Digest

Testdigest

Retrieved from "http://2014.igem.org/Team:Freiburg/Content/Notebook"