Team:Evry/Overview/Project

From 2014.igem.org

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Our synthetic system is Pseudovibrio denitrificans, however its origin from depth oceans make hard its transformation.  
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Our synthetic system is based on the bacterium Pseudovibrio denitrificans, however its origin from depth oceans make hard its transformation.  
In order to be able to transform our bacterium and insert genes of interest, we have introduce a new format :<FONT COLOR=#CC6699> <b>the transposon</b></font>.  
In order to be able to transform our bacterium and insert genes of interest, we have introduce a new format :<FONT COLOR=#CC6699> <b>the transposon</b></font>.  
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This tool allows to insert genes easily into unknown bacteria.
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This tool allows to insert genes easily into unknown bacteria and the presence of an universal ORI enable its replication.  
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Indeed, our construct is compound of a universal ORI.
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Revision as of 18:59, 16 October 2014

IGEM Evry 2014

Overview - Project


The Sponge Patrol Project



Water is the most precious and fragile resource on Earth. However seas and oceans are polluted by a wide range of compounds stemming from human activities. Faced with this issue, our team wants to heighten awareness about environmental pollution and particularly its devastating consequences on the aquatic life. In this line of thinking we are driven to design a bioremediation tool based on the considerable filtration capacity of sponges to decrease the marine pollution and protect aquatic ecosystems. Our project is based on the engineering of this bacterium in the microbiome in order to develop a filtrating system allowing to sense and even degrade pollutants in water





Chassis
Conference
Transposons
Workshops
Target
Hackathons


Chassis


For our third participation to the iGEM competition, we decided to use a new chassis to the competition, which is able to work in depth of oceans through a forceful duo: sponge and bacterium. Sponge can filtrate up to 20m3 of water/day/kg making it one of the most powerful filtrating system alive. We focused on the sponge Spongia Officinalis, which lives in symbiosis with the bacterium: Pseudovibrio denitrificans.
Pseudovibrio denitrificans is a marine bacterium not yet characterize and has never been used in iGEM.
Likewise, we add Spongia Officinalis, a sponge with impressive features and capacities.
This is why we dedicate a part introducing them.



Transposons


Our synthetic system is based on the bacterium Pseudovibrio denitrificans, however its origin from depth oceans make hard its transformation. In order to be able to transform our bacterium and insert genes of interest, we have introduce a new format : the transposon. This tool allows to insert genes easily into unknown bacteria and the presence of an universal ORI enable its replication.



Target


This year, our team objective is to develop a filtrating system which could allow to sense and could even degrade pollutants in the future. For this project, we have chosen to work on elements most great classes of marine pollutants belonging to most important classes of pollutants of the marine environment. Hence, we have chosen to focus on phenols, PCBs, nitrite and heavy metals.