Team:Evry/Overview/Achievements/Projects

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<FONT COLOR="blue"><h2 id="Project">Projects Achievements</h2></FONT>  
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<FONT COLOR="blue"><h2 id="Project"><img src="https://static.igem.org/mediawiki/2014/1/1b/Rightlool.png" width="5%"/>Projects Achievements<img src="https://static.igem.org/mediawiki/2014/4/4d/Right_real.png" width="5%"/></h2></FONT>  
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<FONT color=#003399><h5><b>Chassis characterization</b></h5> </font>
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/61/Cells_pic.png" width="5%"/>Chassis characterization</b></h5> </font>
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<br>  <li>- Genome Sequencing</li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/GenomeAssembly"> Genome Sequencing </a>of <i> Pseudovibrio denitrificans</i> to collect informations defense mechanisms, <br> transposons putative insertion localisation and to possess a assembled genome for RNAseq. </li>  
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<br>  <li>- Growth conditions optimization </li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis">Growth conditions optimization</a> </li>  
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<br>  <li>- Antibiotic sensitivity </li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Antibiotic sensitivity</a> tests shown a potential resistance to tetracycline and ampicilline</li>
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<br>  <li>- Electroporation Transformation protocol </li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Electroporation Transformation </a> protocol establishment that never been done before </li>
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<FONT color=#003399><h5><b>New Vector</b></h5> </font>
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/6e/Plasmid_khd.png" width="5%"/>New Vector</b></h5> </font>
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<br>  <li>- Transposon-based vector </li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Transposons">Transposon-based vector</a> was created to overcome resistance mechanisms to DNA entrance</li>
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<br>  <li>- Proof of concept in <i>Pseudovibrio denitrificans</i> </li>
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<br>  <li>- Proof of concept to use it in <i>Pseudovibrio denitrificans</i> was achieved </li>
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<FONT color=#003399><h5><b>New or Optimized sensors Sensors</b></h5> </font>
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/9/9c/Sensors.png" width="5%"/>New or Optimized sensors Sensors</b></h5> </font>
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<br>  <li>- New PCB sensor </li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> New PCB sensor </a> based on bphR1 promoter and binding protein bphR2.</li>
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<br>  <li>- An optimized version of the Phenol sensors</li>
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<br>  <li>- An optimized version of <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> the Phenol sensors </a> from Pekin iGEM 2013 team was constructed</li>
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<br>  <li>- <a href="https://2014.igem.org/Team:Evry/Biology/RNAseq"> RNA-seq data </a> shall be used to find new promoters</li>  
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Latest revision as of 03:56, 18 October 2014

Projects Achievements



Chassis characterization


New Vector


  • - Transposon-based vector was created to overcome resistance mechanisms to DNA entrance

  • - Proof of concept to use it in Pseudovibrio denitrificans was achieved


New or Optimized sensors Sensors