Team:Evry/Notebook/Transposons/16.10.14
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== Show BBa_1413044 work even empty in DH5alpha == | == Show BBa_1413044 work even empty in DH5alpha == | ||
- | 8 colonies of DH5alpha were transformed with BBa_1413044 + 2 negative control | + | 8 colonies of DH5alpha were transformed with BBa_1413044 and grown in 3 ml LB + 2 negative control |
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+ | Preparation for PCR colony: | ||
+ | Spin down 500 ul culture at 14000 g - 2 min, resuspend in 100 ul H20 MilliQ, put at 95 C° for 10 min then spin down at 14000 g - 2 min. | ||
+ | PCR: enzyme: Q5; template: 3 ul of supernatent for the culture; oligo: F 100/ R 101; Tm tested: 55; elongation time: 1m00s | ||
+ | <img src="" width="50%"/> | ||
Revision as of 21:07, 17 October 2014
== Show BBa_1413044 work even empty in DH5alpha == 8 colonies of DH5alpha were transformed with BBa_1413044 and grown in 3 ml LB + 2 negative control Preparation for PCR colony: Spin down 500 ul culture at 14000 g - 2 min, resuspend in 100 ul H20 MilliQ, put at 95 C° for 10 min then spin down at 14000 g - 2 min. PCR: enzyme: Q5; template: 3 ul of supernatent for the culture; oligo: F 100/ R 101; Tm tested: 55; elongation time: 1m00s
Aug 08