Team:Evry/Notebook/Transposons/16.10.14

From 2014.igem.org

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== Show BBa_1413044 work even empty in DH5alpha ==
== Show BBa_1413044 work even empty in DH5alpha ==
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8 colonies of DH5alpha were transformed with BBa_1413044 + 2 negative control
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8 colonies of DH5alpha were transformed with BBa_1413044 and grown in 3 ml LB + 2 negative control
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<img src="" width="50%"/>
 
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PCR:
 
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Preparation for PCR colony:
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Spin down 500 ul culture at 14000 g - 2 min, resuspend in 100 ul H20 MilliQ, put at 95 C° for 10 min then spin down at 14000 g - 2 min.
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PCR: enzyme: Q5; template: 3 ul of supernatent for the culture; oligo: F 100/ R 101; Tm tested: 55; elongation time: 1m00s
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<img src="" width="50%"/>

Revision as of 21:07, 17 October 2014

Picture

== Show BBa_1413044 work even empty in DH5alpha == 8 colonies of DH5alpha were transformed with BBa_1413044 and grown in 3 ml LB + 2 negative control Preparation for PCR colony: Spin down 500 ul culture at 14000 g - 2 min, resuspend in 100 ul H20 MilliQ, put at 95 C° for 10 min then spin down at 14000 g - 2 min. PCR: enzyme: Q5; template: 3 ul of supernatent for the culture; oligo: F 100/ R 101; Tm tested: 55; elongation time: 1m00s

Aug 08