Team:Evry/Notebook/Transposons/16.10.14

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== Show BBa_1413044 work even empty in DH5alpha==
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It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
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8 clonies + 2 negative control
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<br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.
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<img src="" width="50%"/>
<img src="" width="50%"/>

Revision as of 20:58, 17 October 2014

Picture

== Show BBa_1413044 work even empty in DH5alpha== 8 clonies + 2 negative control

Aug 08

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