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Team:Evry/Notebook/Transposons/15.10.14 - Revision history
2024-03-28T23:33:26Z
Revision history for this page on the wiki
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Chesne j at 23:01, 17 October 2014
2014-10-17T23:01:23Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:01, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div align="center"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="" width="50%"/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> </h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> </h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div align="center"></ins><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div align="center"></ins><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br<ins class="diffchange diffchange-inline">><div align="center"</ins>><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td></tr>
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Chesne j
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=365092&oldid=prev
Rbodinier at 23:00, 17 October 2014
2014-10-17T23:00:42Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:00, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br<ins class="diffchange diffchange-inline">><p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364977&oldid=prev
Rbodinier at 22:59, 17 October 2014
2014-10-17T22:59:32Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br<del class="diffchange diffchange-inline">><p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364931&oldid=prev
Rbodinier at 22:59, 17 October 2014
2014-10-17T22:59:00Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><div align="center"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><img src="" width="50%"/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> </h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> </h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364764&oldid=prev
Rbodinier at 22:57, 17 October 2014
2014-10-17T22:57:21Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:57, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>LB agar for LB for DH5alpha</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Transformation <i>Pseudovibrio denitrificans</i> & Dh5 alpha with BBa_1413001 in BBa_1413044 </h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4> Transformation <i>Pseudovibrio denitrificans</i> & Dh5 alpha with BBa_1413001 in BBa_1413044 </h4></div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364350&oldid=prev
Rbodinier at 22:52, 17 October 2014
2014-10-17T22:52:54Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for Kan gene insertion:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for Kan gene insertion:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br<ins class="diffchange diffchange-inline">></p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br<ins class="diffchange diffchange-inline">><p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br<ins class="diffchange diffchange-inline">></p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br<ins class="diffchange diffchange-inline">><p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br<ins class="diffchange diffchange-inline">></p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364225&oldid=prev
Rbodinier at 22:51, 17 October 2014
2014-10-17T22:51:38Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for Kan gene insertion:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for Kan gene insertion:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br<del class="diffchange diffchange-inline">></p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br<del class="diffchange diffchange-inline">><p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 72/ R 73; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br<del class="diffchange diffchange-inline">></p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/e/e7/Evry_Pseudo_spe.JPG" width="50%"/></div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=364133&oldid=prev
Rbodinier at 22:50, 17 October 2014
2014-10-17T22:50:38Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><span class="cd-date">Oct 15</span> </div></td></tr>
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Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=363984&oldid=prev
Rbodinier at 22:49, 17 October 2014
2014-10-17T22:49:08Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:49, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As a control we tested the specificity of our oligo on the strain we work with:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins><img src="https://static.igem.org/mediawiki/2014/0/0b/Pseudo_spe.JPG"width="50%"/></div></div></td></tr>
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</table>
Rbodinier
http://2014.igem.org/wiki/index.php?title=Team:Evry/Notebook/Transposons/15.10.14&diff=363962&oldid=prev
Rbodinier at 22:48, 17 October 2014
2014-10-17T22:48:54Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:48, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>PCR: enzyme: Q5; template: 200 ng genomic preparation <i>Pseudovibrio denitrificans</i>; oligo: F 82/ R 83; Tm tested: 55; elongation time: 2m00s</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><div align="center"></del><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2014/6/6c/Kan.JPG" width="50%"/></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR for <i>Pseudovibrio denitrificans</i> specific oligo:</div></td></tr>
</table>
Rbodinier