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Team:Evry/Notebook/Transposons/08.19.14
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The promoter front emGFP is inductible by glycerol (8%). So a test of induction has been realized. Different cells is cultivated with LB 1X and glycerol 8% add kanamycin (25µg/mL) for DH5α pir and Bl21 transformed and MB 1X and glycerol 8% add kanamycin (50µg/mL) for PseudoD. | The promoter front emGFP is inductible by glycerol (8%). So a test of induction has been realized. Different cells is cultivated with LB 1X and glycerol 8% add kanamycin (25µg/mL) for DH5α pir and Bl21 transformed and MB 1X and glycerol 8% add kanamycin (50µg/mL) for PseudoD. | ||
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Latest revision as of 20:17, 17 October 2014
Induction of mGFP
The promoter front emGFP is inductible by glycerol (8%). So a test of induction has been realized. Different cells is cultivated with LB 1X and glycerol 8% add kanamycin (25µg/mL) for DH5α pir and Bl21 transformed and MB 1X and glycerol 8% add kanamycin (50µg/mL) for PseudoD.
Aug 22
