Team:Evry/Notebook/Transposons/08.06.14

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It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.  
It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.  
<br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.  
<br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.  
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/>
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/></p>
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<img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/><br/>
<img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/><br/>
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<span class="cd-date">Aug 08</span>  
<span class="cd-date">Aug 08</span>  

Latest revision as of 20:06, 17 October 2014

Picture

It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.


Aug 08