Team:Evry/Notebook/Transposons/08.06.14

From 2014.igem.org

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It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
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<br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/></p>
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<span class="cd-date">Aug 08</span>  
<span class="cd-date">Aug 08</span>  
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Latest revision as of 20:06, 17 October 2014

Picture

It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.


Aug 08