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Team:Evry/Notebook/Transposons/08.06.14
From 2014.igem.org
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It’s the first day or Brian Jester give us the plasmid pNK2. This plasmid contains transposon. | It’s the first day or Brian Jester give us the plasmid pNK2. This plasmid contains transposon. | ||
| - | A Precultur of DH5α pir is realized. In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. | + | <br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein. |
| - | It parallel annotation of plasmid pNK2 with Blast is realized. | + | <br/>It parallel annotation of plasmid pNK2 with Blast is realized. |
<img src="https://2014.igem.org/File:PNK2.png"/> | <img src="https://2014.igem.org/File:PNK2.png"/> | ||
Revision as of 16:18, 17 October 2014
It’s the first day or Brian Jester give us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.
