Team:Evry/Notebook/Transformation/09-09-2014

From 2014.igem.org

(Difference between revisions)
Line 8: Line 8:
<p align="justify">
<p align="justify">
-
<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </b> </u> <br>
+
<u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br>
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour. <br>
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour. <br>

Revision as of 17:08, 12 October 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour.

IMAGE
Gel of digestion with XbaI (2)
There was a problem with the digestion. We are going to try with another enzyme.

Stock of pBBR1MCS and pRhokHI-2
To remake a stock of these plasmids, we showed a colony of E.Coli transformed in a new LB 1X + Cam (1:1000) culture of 3mL.

Sep 09