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Team:Evry/Notebook/Transformation/09-09-2014
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| - | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </b> </u> <br> | + | <u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br> |
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour. <br> | The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour. <br> | ||
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| + | There was a problem with the digestion. We are going to try with another enzyme.<br> | ||
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| - | + | <u> <b> Stock of pBBR1MCS and pRhokHI-2 </b> </u> <br> | |
| - | + | To remake a stock of these plasmids, we showed a colony of E.Coli transformed in a new LB 1X + Cam (1:1000) culture of 3mL.<br> | |
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Latest revision as of 22:01, 17 October 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour.
There was a problem with the digestion. We are going to try with another enzyme.
Stock of pBBR1MCS and pRhokHI-2
To remake a stock of these plasmids, we showed a colony of E.Coli transformed in a new LB 1X + Cam (1:1000) culture of 3mL.
