Team:Evry/Notebook/Transformation/09-09-2014

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There was a problem with the digestion. We are going to try with another enzyme.
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There was a problem with the digestion. We are going to try with another enzyme.<br>
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<u> <b> Stock of pBBR1MCS and pRhokHI-2 </b> </u> <br>
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To remake a stock of these plasmids, we showed a colony of E.Coli transformed in a new LB 1X + Cam (1:1000) culture of 3mL.<br>
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Revision as of 18:16, 11 September 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour.

IMAGE
Gel of digestion with XbaI (2)
There was a problem with the digestion. We are going to try with another enzyme.

Stock of pBBR1MCS and pRhokHI-2
To remake a stock of these plasmids, we showed a colony of E.Coli transformed in a new LB 1X + Cam (1:1000) culture of 3mL.

Sep 09