Revision as of 22:00, 17 October 2014 by Chesne j (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation

We made a restriction control of our plasmid, and we tried to prove that these plasmids are present in our Pseudovibrio.
After made a purification of plasmids in A1 and A2 with NucleoSpin Plamsid Kit (Macherey Nagel), we obtained concentrations at 380.1 ng/µL for pRhokHI-2 and at 91.1 ng/µL for pBBR1MCS.

We disgested these sample and our stock of pRhokHI-2 and pBBR1MCS with XbaI (unique restriction site on these plasmids) and we have make migrate the product of digestion.

Gel of digestion with XbaI

There was a problem with the digestion. We are going to try with a longer time of incubation.

Stock of pBBR1MCS and pRhokHI-2
To remake a stock of these plasmids, we put in liquid LB+Cam (1:1000) culture, a colony from a LB+Cam plate of E.Coli transformed.

Sep 08