Team:Evry/Notebook/Transformation/09-08-2014

From 2014.igem.org

(Difference between revisions)
Line 36: Line 36:
-
There was a problem with the digestion. We are going to try with another enzyme.
+
There was a problem with the digestion. We are going to try with a longer time of incubation.

Revision as of 18:10, 11 September 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
We made a restriction control of our plasmid, and we tried to prove that these plasmids are present in our Pseudovibrio.
After made a purification of plasmids in A1 and A2 with NucleoSpin Plamsid Kit (Macherey Nagel), we obtained concentrations at 380.1 ng/µL for pRhokHI-2 and at 91.1 ng/µL for pBBR1MCS.

We disgested these sample and our stock of pRhokHI-2 and pBBR1MCS with XbaI (unique restriction site on these plasmids) and we have make migrate the product of digestion.

IMAGE
Gel of digestion with XbaI
There was a problem with the digestion. We are going to try with a longer time of incubation.

Sep 08