Team:Evry/Notebook/Transformation/09-02-2014

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Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results

  • Positive control with E.Coli :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

  • Resulst with Pseudovibrio :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

    NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old.

    A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. On Chloramphenicol, the contamination does'nt seem to grow.
    We have to prove that it's Pseudovibrio wich growth on Chloramphenicol.

    Sep 02