Team:Evry/Notebook/Transformation/09-02-2014

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<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results </b> </u> <br>
 
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<li> Positive control with E.Coli : <br>
 
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<img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
 
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<u> <b> <big><FONT COLOR=#003333> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results </font></big></b> </u> <br>
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<li> Positive control with E.Coli : <br>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" style="width:60%;height:60%" /> <br>
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<li> Resulst with Pseudovibrio : <br>
<li> Resulst with Pseudovibrio : <br>
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<img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" style="width:60%;heigth:60%" /> </div><br>
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br>
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br>
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A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000.  
A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000.  
On Chloramphenicol, the contamination does'nt seem to grow. <br>
On Chloramphenicol, the contamination does'nt seem to grow. <br>

Latest revision as of 21:58, 17 October 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results

  • Positive control with E.Coli :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

  • Resulst with Pseudovibrio :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

    NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old.

    A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. On Chloramphenicol, the contamination does'nt seem to grow.
    We have to prove that it's Pseudovibrio wich growth on Chloramphenicol.

    Sep 02