Team:Evry/Notebook/Transformation/09-02-2014

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<li> Positive control with E.Coli : <br>
<li> Positive control with E.Coli : <br>
<img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
<img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
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<br>
<li> Resulst with Pseudovibrio : <br>
<li> Resulst with Pseudovibrio : <br>
<img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
<img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br>
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br>
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br>
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<br>
A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000.  
A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000.  
On Chloramphenicol, the contamination does'nt seem to grow. <br>
On Chloramphenicol, the contamination does'nt seem to grow. <br>

Revision as of 13:22, 9 September 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results

  • Positive control with E.Coli :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

  • Resulst with Pseudovibrio :
    GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE
    NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old.

    A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. On Chloramphenicol, the contamination does'nt seem to grow.
    We have to prove that it's Pseudovibrio wich growth on Chloramphenicol.

    Sep 02