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Team:Evry/Notebook/Sensing/Phenol/08-20-2014
From 2014.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
GFP B0032: 1330 bp <br> | GFP B0032: 1330 bp <br> | ||
<u>Digestion</u> <br> | <u>Digestion</u> <br> | ||
| + | From newly extracted DNA<br> | ||
DmpR: SpeI&PstI<br> | DmpR: SpeI&PstI<br> | ||
GFP B0031/32: XbaI&PstI<br><br> | GFP B0031/32: XbaI&PstI<br><br> | ||
| - | |||
| - | |||
| + | <img src="https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG"><br><br> | ||
| + | <u>Analysis</u><br> | ||
| + | VF2/VR PCR products are still in agreement with the expected size either for DmpR and GFP B0031/32.<br> | ||
| + | GFP 31/32 digestion products were in agreement with the expected size.<br> | ||
| + | DmpR digestion by SP is still displaying 2 close bands profile.<br><br> | ||
</p> | </p> | ||
Revision as of 23:23, 15 September 2014
PCR using VF2 and VR primer
Q5 polymerase
Expected bands :
DmpR: 2038 bp
GFP B0031: 1331 bp
GFP B0032: 1330 bp
Digestion
From newly extracted DNA
DmpR: SpeI&PstI
GFP B0031/32: XbaI&PstI
Analysis
VF2/VR PCR products are still in agreement with the expected size either for DmpR and GFP B0031/32.
GFP 31/32 digestion products were in agreement with the expected size.
DmpR digestion by SP is still displaying 2 close bands profile.
