"
Team:Evry/Notebook/Sensing/Phenol/08-20-2014
From 2014.igem.org
(Difference between revisions)
(Created page with "<html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/Sensing/Phenol/TopImage}} <html> <div class="cd-timeline-content"> <p align="justify"> <u>PCR us...") |
|||
| Line 15: | Line 15: | ||
<u>Digestion</u> <br> | <u>Digestion</u> <br> | ||
DmpR: SpeI&PstI<br> | DmpR: SpeI&PstI<br> | ||
| - | GFP B0031/32: XbaI&PstI<br> | + | GFP B0031/32: XbaI&PstI<br><br> |
https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG | https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG | ||
<img src="https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG"> | <img src="https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG"> | ||
| + | |||
</p> | </p> | ||
Revision as of 23:04, 15 September 2014
PCR using VF2 and VR primer
Q5 polymerase
Expected bands :
DmpR: 2038 bp
GFP B0031: 1331 bp
GFP B0032: 1330 bp
Digestion
DmpR: SpeI&PstI
GFP B0031/32: XbaI&PstI
https://static.igem.org/mediawiki/2014/2/23/EVRY_Dmpr_GFP_GEL_20-08_PNG.PNG
