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Team:Evry/Notebook/Sensing/PCBs/08-15-2014
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| - | + | <u><h4>Sensor construction HbpR/PhbpC/HbpC:</h4></u> | |
| + | Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL | ||
| + | <br/> | ||
| + | <u><h4>Survival test on E.coli BL21:</h4></u> | ||
| + | A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO | ||
| + | <ol> | ||
| + | <li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L | ||
| + | <br/><img src="https://2014.igem.org/File:Evry_2014_PCB_dilution.TIF" alt="image not found"/> | ||
| + | <li> 300µL of E.coli BL21 was added in each eppendorf tube. | ||
| + | <br/> We had 2 control tubes: | ||
| + | <br/><ul> | ||
| + | <li> A negative control: 2,7mL of M9 medium + 300µL of compound | ||
| + | <li> A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21 | ||
| + | </ul> | ||
| + | <li> Incubation of tubes at 37°C overnight, 200rpm | ||
</p> | </p> | ||
Revision as of 10:12, 25 August 2014
Sensor construction HbpR/PhbpC/HbpC:
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
Survival test on E.coli BL21:
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
- Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
- 300µL of E.coli BL21 was added in each eppendorf tube.
We had 2 control tubes:- A negative control: 2,7mL of M9 medium + 300µL of compound
- A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
- Incubation of tubes at 37°C overnight, 200rpm Aug 15
