Team:Evry/Notebook/Sensing/PCBs/08-15-2014

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<u>Sensor construction HbpR/PhbpC/HbpC:</u>
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<br/>Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
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<u>Survival test on E.coli BL21:</u>
<u>Survival test on E.coli BL21:</u>

Revision as of 21:37, 1 September 2014

Picture


Survival test on E.coli BL21:
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO

  1. Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
    image not found
  2. 300µL of E.coli BL21 were added in each eppendorf tube.
    We had 2 control tubes:
    • A negative control: 2,7mL of M9 medium + 300µL of compound
    • A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
  3. Incubation of tubes at 37°C overnight, 200rpm

    Aug 15

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