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Team:Evry/Notebook/Sensing/PCBs/08-15-2014
From 2014.igem.org
(Difference between revisions)
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<u>Sensor construction HbpR/PhbpC/HbpC:</u> | <u>Sensor construction HbpR/PhbpC/HbpC:</u> | ||
| - | Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL | + | <br/>Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL |
| + | <br/> | ||
<br/> | <br/> | ||
<u>Survival test on E.coli BL21:</u> | <u>Survival test on E.coli BL21:</u> | ||
| - | A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO | + | <br/>A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO |
<ol> | <ol> | ||
<li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L | <li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L | ||
<br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/> | <br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/> | ||
| - | <li> 300µL of E.coli BL21 | + | <li> 300µL of E.coli BL21 were added in each eppendorf tube. |
<br/> We had 2 control tubes: | <br/> We had 2 control tubes: | ||
<br/><ul> | <br/><ul> | ||
Revision as of 16:05, 25 August 2014
Sensor construction HbpR/PhbpC/HbpC:
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
Survival test on E.coli BL21:
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
- Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
- 300µL of E.coli BL21 were added in each eppendorf tube.
We had 2 control tubes:- A negative control: 2,7mL of M9 medium + 300µL of compound
- A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
- Incubation of tubes at 37°C overnight, 200rpm Aug 15
