Team:Evry/Notebook/Sensing/PCBs/08-15-2014

From 2014.igem.org

(Difference between revisions)
Line 7: Line 7:
       <p align="justify">
       <p align="justify">
-
<u><h4>Sensor construction HbpR/PhbpC/HbpC:</h4></u>
+
<u>Sensor construction HbpR/PhbpC/HbpC:</u>
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
<br/>
<br/>
-
<u><h4>Survival test on E.coli BL21:</h4></u>
+
<u>Survival test on E.coli BL21:</u>
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
<ol>
<ol>

Revision as of 10:25, 25 August 2014

Picture

Sensor construction HbpR/PhbpC/HbpC: Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
Survival test on E.coli BL21: A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO

  1. Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
    image not found
  2. 300µL of E.coli BL21 was added in each eppendorf tube.
    We had 2 control tubes:
    • A negative control: 2,7mL of M9 medium + 300µL of compound
    • A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
  3. Incubation of tubes at 37°C overnight, 200rpm

    Aug 15