Team:Evry/Notebook/Sensing/PCBs/08-15-2014

From 2014.igem.org

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<u>Sensor construction HbpR/PhbpC/HbpC:</u>
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Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
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<b><u>Survival test on E.coli BL21:</u></b>
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<u>Survival test on E.coli BL21:</u>
 
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A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
 
<ol>
<ol>
<li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
<li>Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
<br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/>
<br/><img src="https://static.igem.org/mediawiki/2014/9/95/Dilution_compose.jpg" alt="image not found"/>
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<li> 300µL of E.coli BL21 was added in each eppendorf tube.  
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<li> 300µL of E.coli BL21 were added in each eppendorf tube.  
<br/> We had 2 control tubes:  
<br/> We had 2 control tubes:  
<br/><ul>
<br/><ul>
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<li> A negative control: 2,7mL of M9 medium + 300µL of compound  
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<li> A negative control which contains 2,7mL of M9 medium + 300µL of compound  
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<li> A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
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<li> A positive control which contains 2,7mL of M9 medium + 300µL of E.coli BL21
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<li> Incubation of tubes at 37°C overnight, 200rpm
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<li> Tubes were incubated at 37°C overnight, 200rpm
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Latest revision as of 10:48, 6 September 2014

Picture


Survival test on E.coli BL21:

  1. Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
    image not found
  2. 300µL of E.coli BL21 were added in each eppendorf tube.
    We had 2 control tubes:
    • A negative control which contains 2,7mL of M9 medium + 300µL of compound
    • A positive control which contains 2,7mL of M9 medium + 300µL of E.coli BL21

  3. Tubes were incubated at 37°C overnight, 200rpm

    Aug 15