"
Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
<div class="cd-timeline-block"> | <div class="cd-timeline-block"> | ||
</html> | </html> | ||
| - | {{:Team:Evry/Notebook/CellCharacterization/ | + | {{:Team:Evry/Notebook/CellCharacterization/Transformation/TopImage}} |
<html> | <html> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
<p align="justify"> | <p align="justify"> | ||
| + | |||
<u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 </b> </u> <br> | <u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 </b> </u> <br> | ||
Revision as of 14:21, 29 August 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate) (LIEN PROTO).
After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight. Aug 23
