Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014

From 2014.igem.org

(Difference between revisions)
Line 21: Line 21:
</p>
</p>
-
<span class="cd-date">Aug 24</span>  
+
<span class="cd-date">Aug 23</span>  
</div>
</div>
</div>
</div>
</html>
</html>

Revision as of 16:34, 28 August 2014

Picture

Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)

pBBR1MCS pRhokH1-2

Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate).
After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight.

Aug 23