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Team:Evry/Notebook/CellCharacterization/Antibiotic test
From 2014.igem.org
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| - | + | <h4 class=title4> <FONT COLOR="blue">Preparation of antibiotic stocks </FONT></h4> | |
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| - | <td> <b>Antibiotic </b> </td> | + | <p> |
| - | <td> <b>Stock concentration </b> </td> | + | <table border="1"> |
| - | <td> <b>Protocol </b> </td> | + | <tr > |
| + | <td> <b><FONT COLOR=#000033>Antibiotic</font> </b> </td> | ||
| + | <td> <b><FONT COLOR=#000033>Stock concentration</font> </b> </td> | ||
| + | <td class= table> <b><FONT COLOR=#000033>Protocol </font></b> </td> | ||
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<td>Kanamycin </td> | <td>Kanamycin </td> | ||
<td class= conc> 25 mg/mL </td> | <td class= conc> 25 mg/mL </td> | ||
| - | <td> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL)</td> | + | <td class= proto> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) <br> </br></td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td> Streptomycin </td> | <td> Streptomycin </td> | ||
<td class= conc> 100 mg/mL </td> | <td class= conc> 100 mg/mL </td> | ||
| - | <td> Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.<br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) </td> | + | <td class= proto> Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.<br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) <br> </br></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Amplicilin </td> | <td> Amplicilin </td> | ||
| - | <td class= conc> | + | <td class= conc> 100 mg/mL </td> |
| - | <td> Weight 1g of Amplicilin powder, solubilization into | + | <td class= proto> Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) <br> </br> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Tetracyclin</td> | <td> Tetracyclin</td> | ||
<td class= conc> 15 mg/mL </td> | <td class= conc> 15 mg/mL </td> | ||
| - | <td> Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) </td> | + | <td class= proto> Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL)<br> </br> </td> |
</tr> | </tr> | ||
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<td> Chloramphenicol </td> | <td> Chloramphenicol </td> | ||
<td class= conc> 34 mg/mL </td> | <td class= conc> 34 mg/mL </td> | ||
| - | <td> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) | + | <td class= proto> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) <br> </br> </td> |
</tr> | </tr> | ||
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</table> | </table> | ||
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Latest revision as of 10:40, 17 October 2014
Preparation of antibiotic stocks
| Antibiotic | Stock concentration | Protocol |
| Kanamycin | 25 mg/mL | Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Streptomycin | 100 mg/mL | Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Amplicilin | 100 mg/mL | Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Tetracyclin | 15 mg/mL | Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
| Chloramphenicol | 34 mg/mL | Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
