Team:Evry/Notebook/CellCharacterization/Antibiotic test

From 2014.igem.org

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<h4 class=title4> <FONT COLOR="blue">Preparation of antibiotic stocks </FONT></h4>
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<h4 class=title4> <FONT COLOR=#0099CC><u> Preparation of antibiotic stocks </u> </font></h4>
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  <td> <b><FONT COLOR=#000033>Antibiotic</font> </b> </td>
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  <td> <b>Antibiotic </b> </td>
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   <td> <b><FONT COLOR=#000033>Stock concentration</font> </b> </td>
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   <td> <b>Stock concentration </b> </td>
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   <td class= table> <b><FONT COLOR=#000033>Protocol </font></b> </td>
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   <td> Amplicilin </td>
   <td> Amplicilin </td>
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   <td class= conc> 50 mg/mL </td>
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   <td class= conc> 100 mg/mL </td>
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   <td class= proto> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br> </td>
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   <td class= proto> Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br> </td>
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<span class="cd-date">July 07</span>
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Latest revision as of 10:40, 17 October 2014

Preparation of antibiotic stocks

Antibiotic Stock concentration Protocol
Kanamycin 25 mg/mL Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Streptomycin 100 mg/mL Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Amplicilin 100 mg/mL Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Tetracyclin 15 mg/mL Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)
Chloramphenicol 34 mg/mL Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)

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