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Team:Evry/Biology/Chassis/Pseudovibrio
From 2014.igem.org
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| - | == <b><big>From a poorly known genius | + | == <b><big>From a poorly known genius, a jewel emerged</big></b> == |
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| - | With only 12 species in the genius, and 2 strains sequenced (Pseudovibrio sp. FO-BEG1, Pseudovibrio sp. JE062) we knew we would have to sequence our strain of <i>Pseudovibrio denitrificans </i> with a mapping to one of the 2 strains references. | + | With only 12 species in the genius, and 2 strains sequenced (Pseudovibrio sp. FO-BEG1, Pseudovibrio sp. JE062) we knew we would have to sequence our strain of <i>Pseudovibrio denitrificans </i> with a mapping to one of the 2 strains references. Eventhough in the first place the species was chosen because of its natural denitrification ability (cf <b><big> toxic compound</big></b>) and sourced at <b><big>DMSZ</big></b>. |
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| + | Moreover <i>Pseudovibrio denitrificans </i> or strains related to the species has been shown as majoritary in at least six microbiomes of sponges in the mediterrean sea, where spongia officinalis reside. | ||
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| + | == <b><big>A bacterium we could work with</big></b> == | ||
| + | They were Gram-negative. Cells in late exponential to early stationary phase of growth were predominantly straight or curved rods, but Y- or V-shaped forms were also observed. They were motile by means of one to several lateral or subpolar flagella. Both strains required NaCl for growth and exhibited optimal growth at about 30 degrees C, pH 8 and 3 % NaCl. They were capable of anaerobic growth by carrying out denitrifying metabolism using nitrate, nitrite or nitrous oxide as terminal electron acceptors or, alternatively, by fermenting glucose, mannose, sucrose or trehalose as substrates. Anaerobic fermentative growth on glucose resulted in formation of various organic acids, including formate, lactate, acetate, pyruvate and fumarate | ||
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Revision as of 14:42, 12 October 2014
Pseudovibrio denitrificans the bacterium to modify.
== From a poorly known genius, a jewel emerged ==
With only 12 species in the genius, and 2 strains sequenced (Pseudovibrio sp. FO-BEG1, Pseudovibrio sp. JE062) we knew we would have to sequence our strain of Pseudovibrio denitrificans with a mapping to one of the 2 strains references. Eventhough in the first place the species was chosen because of its natural denitrification ability (cf toxic compound) and sourced at DMSZ.
Moreover Pseudovibrio denitrificans or strains related to the species has been shown as majoritary in at least six microbiomes of sponges in the mediterrean sea, where spongia officinalis reside.
== A bacterium we could work with ==
They were Gram-negative. Cells in late exponential to early stationary phase of growth were predominantly straight or curved rods, but Y- or V-shaped forms were also observed. They were motile by means of one to several lateral or subpolar flagella. Both strains required NaCl for growth and exhibited optimal growth at about 30 degrees C, pH 8 and 3 % NaCl. They were capable of anaerobic growth by carrying out denitrifying metabolism using nitrate, nitrite or nitrous oxide as terminal electron acceptors or, alternatively, by fermenting glucose, mannose, sucrose or trehalose as substrates. Anaerobic fermentative growth on glucose resulted in formation of various organic acids, including formate, lactate, acetate, pyruvate and fumarate
- be massively present on the sponge surface / avoiding being in an unfavorable position for food competition.
- be found mainly in sponges / avoid spreading to species in contact with sponges.
- be the phenotypically closest possible to a known bacterium. / avoid cell cultures difficulties.
