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Team:Evry/Biology/CellCharacterization/Culture
From 2014.igem.org
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Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the <a id="igem-link" target="_blank" href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1813&ID=1918&Menu=1&Item=21.2.10.1.1 " title="Go to iGEM site">TECAN infiniteM200</a>. <br> | Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the <a id="igem-link" target="_blank" href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1813&ID=1918&Menu=1&Item=21.2.10.1.1 " title="Go to iGEM site">TECAN infiniteM200</a>. <br> | ||
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| + | <img src="" alt="Curve TECAN" /><br> | ||
| + | <b><u>Figure2:</u></b> Curve of growth in liquid M9+3%NaCl 1X.<br> | ||
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| + | The marine broth is more complete than the M9+3%NaCl, even if we have adjusted the salinity. As we can see in the Table2, <i>Pseudovibrio denitrificans</i> grows better on MB media. The number of colony is higher, and they are significantly bigger (Figure1).<br> | ||
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Revision as of 13:31, 14 October 2014
Cell culture
With the help of the 'Molecules of defence and communication in the microbial ecosystems' team of the National museum of natural history of Paris (their web page here), we succeed to cultivate our bacteria Pseudovibrio denitrificans in two medium, the marine broth and the minimal medium M9 added by 3% of NaCl.
Table1: Composition of used media, marine broth and M9+3% NaCl.
Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the TECAN infiniteM200.
Figure2: Curve of growth in liquid M9+3%NaCl 1X.
The marine broth is more complete than the M9+3%NaCl, even if we have adjusted the salinity. As we can see in the Table2, Pseudovibrio denitrificans grows better on MB media. The number of colony is higher, and they are significantly bigger (Figure1).
Table2: Number of CFU on plates of MB 1X and M9+3%NaCl 1X.
![Number of CFU on plates of MB 1X and M9+3%NaCl 1X]()
Figure2: Photos of MB 1X and M9+3%NaCl 1X plates.
