http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&feed=atom&action=historyTeam:ETH Zurich/project/background/biotoolssimple - Revision history2024-03-29T05:36:59ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=366578&oldid=prevLnadine: /* Integrases – Molecules working as scissors on the DNA */2014-10-17T23:14:49Z<p><span class="autocomment">Integrases – Molecules working as scissors on the DNA</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:14, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 24:</td>
<td colspan="2" class="diff-lineno">Line 24:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:ETHZ Integrasesites.png|center|300px|thumb|Specific sites for the binding of integrases]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:ETHZ Integrasesites.png|center|300px|thumb|<ins class="diffchange diffchange-inline">'''Figure 5''' </ins>Specific sites for the binding of integrases]]</div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=366559&oldid=prevLnadine: /* Quorum sensing – Communication using signal molecules */2014-10-17T23:14:35Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:14, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|<ins class="diffchange diffchange-inline">'''Figure 4''' </ins>Simplified Quorum Sensing System]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Integrases – Molecules working as scissors on the DNA'''===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Integrases – Molecules working as scissors on the DNA'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=366477&oldid=prevLnadine: /* Biological Tools */2014-10-17T23:13:54Z<p><span class="autocomment">Biological Tools</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:13, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Biological Tools ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Biological Tools ==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==='''Integrases – Molecules working as scissors on the DNA'''===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Integrases are proteins that work like scissors on two specific sites on the DNA. Within those two sites they can invert the sequence or they can completely remove it. The genes coding for those integrase-scissors and their corresponding specific sites can easily be inserted into bacteria. This way you can control which genes are expressed by defining integrases present in your system. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[File:ETHZ Integrasesites.png|center|300px|thumb|Specific sites for the binding of integrases]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Quorum sensing – Communication using signal molecules'''===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Quorum sensing – Communication using signal molecules'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 25:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==='''Integrases – Molecules working as scissors on the DNA'''===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Integrases are proteins that work like scissors on two specific sites on the DNA. Within those two sites they can invert the sequence or they can completely remove it. The genes coding for those integrase-scissors and their corresponding specific sites can easily be inserted into bacteria. This way you can control which genes are expressed by defining integrases present in your system. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[File:ETHZ Integrasesites.png|center|300px|thumb|Specific sites for the binding of integrases]]</ins></div></td></tr>
</table>Lnadinehttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214430&oldid=prevClormeau: /* Quorum sensing – Communication using signal molecules */2014-10-13T14:23:16Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:23, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 22:</td>
<td colspan="2" class="diff-lineno">Line 22:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214428&oldid=prevClormeau: /* Integrases – Molecules working as scissors on the DNA */2014-10-13T14:23:07Z<p><span class="autocomment">Integrases – Molecules working as scissors on the DNA</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:23, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 6:</td>
<td colspan="2" class="diff-lineno">Line 6:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETHZ Integrasesites.png|center|300px|thumb|Specific sites for the binding of integrases]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETHZ Integrasesites.png|center|300px|thumb|Specific sites for the binding of integrases]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214425&oldid=prevClormeau: /* Integrases – Molecules working as scissors on the DNA */2014-10-13T14:22:48Z<p><span class="autocomment">Integrases – Molecules working as scissors on the DNA</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:22, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Integrases – Molecules working as scissors on the DNA'''===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Integrases – Molecules working as scissors on the DNA'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Integrases are proteins that work like scissors on two specific sites on the DNA.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Integrases are proteins that work like scissors on two specific sites on the DNA. <ins class="diffchange diffchange-inline">Within those two sites they can invert the sequence or they can completely remove it. The genes coding for those integrase-scissors and their corresponding specific sites can easily be inserted into bacteria. This way you can control which genes are expressed by defining integrases present in your system. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Within those two sites they can invert the sequence or they can completely remove it. The genes coding for those integrase-scissors and their corresponding specific sites can easily be inserted into bacteria. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">This way you can control which genes are expressed by defining integrases present in your system. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project integrases working as scissors for DNA is one of the core components we tried to implement in Mosaicoli.</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214419&oldid=prevClormeau: /* Quorum sensing – Communication using signal molecules */2014-10-13T14:22:17Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:22, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of their population. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of their population. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI. This way, the sensor cells will also act as a producer cell and trasnfer the signal to the following cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI. This way, the sensor cells will also act as a producer cell and trasnfer the signal to the following cells.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214412&oldid=prevClormeau: /* Quorum sensing – Communication using signal molecules */2014-10-13T14:21:50Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:21, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of their population. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of their population. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI. This way, the sensor cells will also act as a producer cell and trasnfer the signal to the following cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI. This way, the sensor cells will also act as a producer cell and trasnfer the signal to the following cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214404&oldid=prevClormeau: /* Quorum sensing – Communication using signal molecules */2014-10-13T14:21:33Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:21, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells). </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI, <del class="diffchange diffchange-inline">which </del>will <del class="diffchange diffchange-inline">itself trigger </del>the <del class="diffchange diffchange-inline">production of more AHL diffusing </del>to the <del class="diffchange diffchange-inline">next </del>cells. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This activated LuxR protein binds the DNA in the receiver cells and triggers the production of LuxI<ins class="diffchange diffchange-inline">. This way</ins>, <ins class="diffchange diffchange-inline">the sensor cells </ins>will <ins class="diffchange diffchange-inline">also act as a producer cell and trasnfer </ins>the <ins class="diffchange diffchange-inline">signal </ins>to the <ins class="diffchange diffchange-inline">following </ins>cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are also ther systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/project/background/biotoolssimple&diff=214355&oldid=prevClormeau: /* Quorum sensing – Communication using signal molecules */2014-10-13T14:17:38Z<p><span class="autocomment">Quorum sensing – Communication using signal molecules</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:17, 13 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Quorum sensing – Communication using signal molecules'''===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==='''Quorum sensing – Communication using signal molecules'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of <del class="diffchange diffchange-inline">the </del>population <del class="diffchange diffchange-inline">of bacteria</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Bacteria can interact with their surroundings and neighboring cells via cell-to-cell communication. Using this communication they can coordinate the expression of genes and thus the overall behavior of <ins class="diffchange diffchange-inline">their </ins>population. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis<del class="diffchange diffchange-inline">, secretion </del>and diffusion of small molecules, which are able to trigger a response. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Quorum sensing describes a system including a stimulus and a response. It is the synthesis and diffusion of small molecules, which are able to trigger a response<ins class="diffchange diffchange-inline">. One of these small molecules is called N-(3-oxohexanoyl)-L-homoserine lactones, which we will just call AHL here, and is produced by the enzyme LuxI (Please consider the figure below for the flow of AHL between cells)</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Already </del>in <del class="diffchange diffchange-inline">the early 1980s by investigating a specific strain </del>of <del class="diffchange diffchange-inline">bacteria it was found that depending on </del>the cell <del class="diffchange diffchange-inline">density </del>and <del class="diffchange diffchange-inline">the concentration of signal molecules, bacteria were able to communicate</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The AHL produced </ins>in <ins class="diffchange diffchange-inline">one cell can then diffuse out </ins>of the cell and <ins class="diffchange diffchange-inline">reach other cells</ins>. <ins class="diffchange diffchange-inline">In those other cells it binds </ins>and <ins class="diffchange diffchange-inline">activates </ins>a <ins class="diffchange diffchange-inline">protein called LuxR</ins>. <ins class="diffchange diffchange-inline">This activated LuxR protein binds </ins>the <ins class="diffchange diffchange-inline">DNA in the receiver cells and triggers the production of LuxI</ins>, which will <ins class="diffchange diffchange-inline">itself trigger </ins>the <ins class="diffchange diffchange-inline">production </ins>of <ins class="diffchange diffchange-inline">more </ins>AHL <ins class="diffchange diffchange-inline">diffusing to the next </ins>cells. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The communication was visualized by bioluminescence, which is the production </del>and <del class="diffchange diffchange-inline">emission of light by </del>a <del class="diffchange diffchange-inline">living organism</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">It was then found that </del>the <del class="diffchange diffchange-inline">molecule inducing this bioluminescence is N-(3-oxohexanoyl)-L-homoserine lactone</del>, which <del class="diffchange diffchange-inline">we </del>will <del class="diffchange diffchange-inline">just call AHL here and is produced by </del>the <del class="diffchange diffchange-inline">enzyme Luxl (Please consider the figure below for the flow </del>of AHL <del class="diffchange diffchange-inline">between </del>cells<del class="diffchange diffchange-inline">)</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Another enzyme named LuxI produces this AHL. The AHL produced in one cell can then diffuse out of the cell and reach other cells. In those other cells it binds and activates a protein called LuxR. This LuxR protein regulates the gene transcription (first step on the way from DNA to proteins).</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">There are also ther </ins>systems of AHLs corresponding to the LuxR/LuxI system. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Further research on such signal molecules was conducted and other </del>systems of AHLs corresponding to the LuxR/LuxI system <del class="diffchange diffchange-inline">were found</del>. These additional systems are called LasR/LasI and RhlR/RhlI.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:ETH Zurich 2014 Simplified biotools QS.png|center|600px|thumb|Simplified Quorum Sensing System]]</div></td></tr>
</table>Clormeau